Due to its potential as a source of milk, dairy products and meat, buffalo (Bubalus bubalis) breeding has gained ground in Mexico in the last 5 years in the livestock industry (Mota-Rojas et al., Reference Mota-Rojas, Bragaglio, Braghieri, Domínguez-Oliva, Mora-Medina, Álvarez-Macías, Rosa, José and Barile2022). Their climate adaptability, increased resistance to tropical livestock illnesses and improved use of lower quality fodder compared to cattle are advantages of these animals (Torres-Chable et al., Reference Torres-Chable, Ojeda-Robertos, Chay-Canul, Peralta-Torres, Luna-Palomera, Brisdis-Vazquez, Blitvich, Machain-Williams, García-Rejon, Baak-Baak, Dorman and Alegria-Lopez2017). In Mexico, water buffalo have been introduced in regions with warm and humid climates, mainly in the states of Veracruz, Tabasco, Chiapas and Campeche, regions that naturally have large swamps (Peralta-Torres et al., Reference Peralta-Torres, Torres-Chable, Segura-Correa, Ojeda-Roberto, Chay-Canul, Luna-Palomera, Severino-Lendechy and Aké-Villanueva2020). Although farmers believe that buffalo farming is a viable business, there is a knowledge gap in science regarding the factors that affect animal productivity (Hernández-Herrera et al., Reference Hernández-Herrera, Lara-Rodríguez, Vázquez-Luna, Acar-Martínez, Fernández-Figueroa and Velásquez-Silvestre2018).
The production and quality of buffalo milk have stood out as a promising alternative in the dairy industry. These animals are known for their adaptability to different environments and their ability to produce nutrient-rich milk, particularly with high levels of fat, protein and total solids, giving it a creamy texture and distinctive flavor (Rangel et al., Reference Rangel, Oliveira, Araújo, Bezerra, Medeiros, Lima Júnior and Araújo2011; Ahmad and Saleem, Reference Ahmad and Saleem2020). Furthermore, the composition of buffalo milk is notably different from cow's milk, making it an excellent choice for the production of special cheeses and yogurts (Sales et al., Reference Sales, Urbano, Lima Júnior, Galvão Júnior, Brito, Cipolat-Gotet, Borba and Rangel2021).
Body weight (BW) is an important factor within production systems as it influences several other economic characteristics (Ramos-Zapata et al., Reference Ramos-Zapata, Dominguez-Madrigal, García-Herrera, Camacho-Pérez, Lugo-Quintal, Tyasi, Gurgel, Ítavo and Chay-Canul2023; Ruiz-Ramos et al., Reference Ruiz-Ramos, Torres-Chable, Peralta-Torres, Ojeda-Robertos, Luna-Palomera, Portillo-Salgado, Tyasi, Gurgel, Ítavo and Chay-Canul A2023). However, the buffalo production chain is characterized by poor infrastructure investment, generally including the lack of a scale to determine the animal's weight. Biometric measurements of buffalo can be used to estimate BW in a simple and inexpensive way (Ramos-Zapata et al., Reference Ramos-Zapata, Dominguez-Madrigal, García-Herrera, Camacho-Pérez, Lugo-Quintal, Tyasi, Gurgel, Ítavo and Chay-Canul2023; Ruiz-Ramos et al., Reference Ruiz-Ramos, Torres-Chable, Peralta-Torres, Ojeda-Robertos, Luna-Palomera, Portillo-Salgado, Tyasi, Gurgel, Ítavo and Chay-Canul A2023). In this sense, Herrera-López et al. (Reference Herrera-López, García-Herrera, Chay-Canul, González-Ronquillo, Macías-Cruz, Díaz-Echeverría, Casanova-Lugo and Piñeiro-Vázquez2018) and Alejandro-Zarate et al. (Reference Alejandro-Zarate, Salazar-Cuytun, Herrera-Camacho, Cruz-Hernández, Barrientos-Medina, Ptáček, Vargas-Bello-Pérez and Chay-Canul2023) found that hip width (HW) is highly related to BW in crossbred heifers. Mathematical models created using biometric data can be safely used to predict BW (Chico-Alcudia et al., Reference Chico-Alcudia, Portillo-Salgado, Camacho-Perez, Peralta-Torres, Munoz-Benitez, Lendechy, Gurgel, Difante, Ítavo and Chay-Canul2022).
This measurement has an advantage over others since it is simpler to produce and requires less handling of the animal, making it a valuable substitute that can be used without special facilities for restraint and handling of cattle. It can be easily used in any routine practice (Herrera-López et al., Reference Herrera-López, García-Herrera, Chay-Canul, González-Ronquillo, Macías-Cruz, Díaz-Echeverría, Casanova-Lugo and Piñeiro-Vázquez2018). In view of this, we examined the hypothesis that HW can be used to predict BW in dairy buffaloes reared in tropical environments. Therefore, the objectives of this study were to understand the relationship between HW and BW and to develop equations to predict BW based on HW in replacement dairy buffaloes reared in tropical environments.
Materials and methods
The buffalo were managed in accordance with the ethical guidelines and animal experimentation regulations of the Department of Agricultural Sciences of the Universidad Juárez Autónoma de Tabasco (approval code: UJAT-2012-IA-18) on a commercial farm located in Isla, Veracruz State, Mexico.
The climate of the region is warm and humid with summer rains and a mean annual temperature and rainfall of 25°C and 2750 mm, respectively. Body weight (BW, kg) and hip width (HW, cm) data were obtained for 215 Murrah buffaloes aged 3 months to 5 years. The animals were raised in extensive pasture production systems with native trees, shrubs, grasses, herbs, and aquatic vegetation (Ramos-Zapata et al., Reference Ramos-Zapata, Dominguez-Madrigal, García-Herrera, Camacho-Pérez, Lugo-Quintal, Tyasi, Gurgel, Ítavo and Chay-Canul2023). Water was provided ad libitum. None of the animals received supplements (Ramos-Zapata et al., Reference Ramos-Zapata, Dominguez-Madrigal, García-Herrera, Camacho-Pérez, Lugo-Quintal, Tyasi, Gurgel, Ítavo and Chay-Canul2023; Ruiz-Ramos et al., Reference Ruiz-Ramos, Torres-Chable, Peralta-Torres, Ojeda-Robertos, Luna-Palomera, Portillo-Salgado, Tyasi, Gurgel, Ítavo and Chay-Canul A2023). The BW was recorded by weighing the animals on a fixed platform scale with a capacity of 2000 kg and an accuracy of 0.5 kg, while HW was recorded using a 65 cm forceps (Haglöf®).
For the statistical analysis and internal validation of the model, the data were processed in the Python environment as follows: descriptive statistics were obtained using the ‘description’ function of the ‘pandas’ package. The ratio between HW and BW was determined by linear (Eq. 1), quadratic (Eq. 2), and allometric (Eq. 3) equations using the ‘lmfit’ package. The following allometric equation was fitted: Y = aX**b, where Y represents BW, X represents HW, and a and b are parameters of the model. The models and their residuals were plotted with the ‘matplotlib’ package. The goodness-of-fit of the regression models was evaluated using the Akaike information criterion (AIC), the Bayesian information criterion (BIC), the coefficient of determination (R 2), the mean square error (MSE), and the root MSE (RMSE). The last three parameters were obtained using the ‘scikit-learn’ package.
The predictive capacity of the three models for BW was evaluated by cross-validating k-folds (k = 4). This approach involved randomly dividing the set of observation values into non-overlapping k-folds of approximately the same size. The first fold was treated as a validation set, and the model fit the remaining k − 1 folds (training data). The ability of the fitted model to predict the actual observed values was evaluated using MSE, R 2, and the mean absolute error (MAE). The mean absolute error is an alternative to the mean squared prediction error (MSPE) that is less sensitive to outliers and is related to the mean absolute difference between observed and predicted results. Lower values of root MSPE and MAE indicate a better fit. The k-folds cross-validation was performed using the ‘scikit-learn’ package, which allowed a comparison of numerous multivariate calibration models.
Results
The 215 animals evaluated had a body weight (BW, mean ± sd) of 341 ± 162 kg, ranging from 58 to 654 kg, while the hip width (HW) was 47.5 ± 12.3 cm, with a minimum of 22 cm and a maximum of 68 cm. The BW and HW were positively and highly correlated (r = 0.97; P < 0.001).
The regression equations describing the estimation of BW according to the three models are presented in Table 1, and the data are plotted in Fig. 1. This figure illustrates external validation, which consisted of evaluating observed vs. predicted values of the three proposed models. The quadratic model was superior to the linear and allometric models in terms of MSE, RMSE, and AIC values. The quadratic model had the lowest MSE (1228.64) and RMSE (35.05). This model also had the lowest AIC (1532.41), although a larger BIC (1542.52). However, the coefficient of determination (R 2 = 0.95) was the same for all models.
Regression equations to estimate body weight (kg) of replacement dairy buffaloes kept under humid tropical conditions
BW, body weight; HW, hip width; N, number of observations; R 2, Coefficient of determination; MSE, mean square error; RMSE, Root MSE; AIC, Akaike Information Criterion; BIC, Bayesian Information Criterion. Values in parentheses are the parameter estimates' standard errors (se) The * indicates: *: P < 0.05; **: P < 0.01; ***: P < 0.001.
Body weight (BW) prediction equations using the Hip width (HW) in replacement dairy buffaloes raised in tropical humid conditions (n = 215).
The linear model exhibited smaller values than the allometric model but greater values than the quadratic model. The quadratic model displayed improved goodness-of-fit scores in relation to the validation criteria, presenting better predictive performance. The quality of fit using the k-folds technique (cross-validation) allowed us to identify that the three proposed models showed an adequate fit considering the internal validation (Table 2). Among these, the quadratic and allometric models had lower values of MSEP (34.59) and MAE (2686) and a high coefficient of determination (R 2 = 0.95). The quadratic model showed a high predictive capacity for body weight using HW as the only predictor in dairy buffaloes (Fig. 1).
Internal k-folds cross-validation of the proposed models
MSPE, mean squared prediction error; R 2, coefficient of determination; MAE, mean absolute error.
Discussion
The findings revealed a robust positive correlation between BW and HW in dairy buffaloes reared in tropical environments aged 3 months to 5 years. Franco et al. (Reference Franco, Marcondes, Campos, Freitas, Detmann and Valadares-Filho2017) reported r = 0.88 and R 2 = 0.83 between BW and HW in Holstein crossbred heifers; these authors concluded that although HW was highly correlated with BW, it showed a low R 2 with a high coefficient of variation in relation to other body measurements. However, our relationship between BW and HW was high, with a higher value (r = 0.97), which is similar to that reported in tropical replacement heifers (Herrera-López et al., Reference Herrera-López, García-Herrera, Chay-Canul, González-Ronquillo, Macías-Cruz, Díaz-Echeverría, Casanova-Lugo and Piñeiro-Vázquez2018; Alejandro-Zarate et al., Reference Alejandro-Zarate, Salazar-Cuytun, Herrera-Camacho, Cruz-Hernández, Barrientos-Medina, Ptáček, Vargas-Bello-Pérez and Chay-Canul2023). This higher relationship may be due, at least in part, to the age (and hence size) range of our study population. The body weight of adult female Murrah buffaloes is highly correlated with body measurements (Ruiz-Ramos et al., Reference Ruiz-Ramos, Torres-Chable, Peralta-Torres, Ojeda-Robertos, Luna-Palomera, Portillo-Salgado, Tyasi, Gurgel, Ítavo and Chay-Canul A2023). Possibly, HW is a good predictor variable of BW because it indicates the development of the skeletal structure in animals, and this body measurement is located in the hindquarters, exactly where the highest body weight of the animal is concentrated (Bretschneider et al., Reference Bretschneider, Cuatrin, Arias and Vottero2014; Herrera-López et al., Reference Herrera-López, García-Herrera, Chay-Canul, González-Ronquillo, Macías-Cruz, Díaz-Echeverría, Casanova-Lugo and Piñeiro-Vázquez2018). Thus, a wider hip would be expected to support a greater accumulation of muscle and fat in this body region, positively favoring the change in BW, as suggested by the developed equation. Therefore, anatomical measurements as indicators of skeletal size may reflect the true size of replacement females (heifers) and consequently their BW (Herrera-López et al., Reference Herrera-López, García-Herrera, Chay-Canul, González-Ronquillo, Macías-Cruz, Díaz-Echeverría, Casanova-Lugo and Piñeiro-Vázquez2018).
The size or frame of the cow is an important trait for dairy cattle, as these dimensions can influence milk yield and intake capacity (Williams et al., Reference Williams, Parsons, Dafoe, Boss, Bowman and Curto2018). For cows of different size but equivalent milk yield, the smaller the animal, the greater her gross efficiency. An animal's weight is defined by the quantity of tissues, including the skeleton, which is in turn affected by both long-term growth and short-term changes in energy balance during lactation. An animal's frame is dictated by its skeletal size, which increases as the animal ages, as our results show. According to Ruiz-Ramos et al. (Reference Ruiz-Ramos, Torres-Chable, Peralta-Torres, Ojeda-Robertos, Luna-Palomera, Portillo-Salgado, Tyasi, Gurgel, Ítavo and Chay-Canul A2023), withers height, rump height, body height, heart girth, abdominal girth, pelvic girth and body length are all parameters that can be used in models to measure the body weight of mature female Murrah buffaloes.
After confirming the correlation between variables, the development of mathematical equations becomes feasible with the goal of elucidating the relationship between predicted and predictor variables. Biometric measurements, as highlighted by Gurgel et al. (Reference Gurgel, Difante, Ítavo, Emerenciano Neto, Ítavo, Fernandes, Costa, Roberto and Chay-Canul2023), have been extensively employed for estimating body weight in ruminant animals. Hence, selecting the mathematical model that most accurately captures this relationship is pivotal, given that the association between live weight and biometric measurements does not consistently follow a linear pattern (Salazar-Cuytun et al., Reference Salazar-Cuytun, Portillo-Salgado, García-Herrera, Camacho-Pérez, Zaragoza-Vera, Gurgel, Muñoz-Osorio and Chay-Canul2022; Ramos-Zapata et al., Reference Ramos-Zapata, Dominguez-Madrigal, García-Herrera, Camacho-Pérez, Lugo-Quintal, Tyasi, Gurgel, Ítavo and Chay-Canul2023). Consequently, a critical phase in modeling studies involves the thorough evaluation of the equations (Tedeschi, Reference Tedeschi2006).
The model evaluation is performed to assess the degree of robustness based on predefined criteria. It is crucial to emphasize that a combination of statistical methods must be employed to collectively evaluate whether the equation is suitable for its intended purpose, considering specific conditions (Gurgel et al., Reference Gurgel, Difante, Ítavo, Emerenciano Neto, Ítavo, Fernandes, Costa, Roberto and Chay-Canul2023). The use of isolated measures (e.g., R 2) is often misinterpreted, as these criteria primarily measure precision rather than accuracy, as highlighted by Tedeschi (Reference Tedeschi2006). The values of BIC, AIC, and RMSE indicate a model within a set of evaluated models that minimizes errors (Tedeschi, Reference Tedeschi2006).
The analysis of this set of criteria, which assesses the quality of model fit, revealed that the quadratic and allometric models provide more precise and accurate estimates of body weight when using HW as the sole predictor. Similarly, Ramos-Zapata et al. (Reference Ramos-Zapata, Dominguez-Madrigal, García-Herrera, Camacho-Pérez, Lugo-Quintal, Tyasi, Gurgel, Ítavo and Chay-Canul2023) explored the hypothesis that body volume measurement could be used as the sole predictor of BW in buffaloes, recommending quadratic and allometric models. Despite our research confirming the tested hypothesis, we encourage further studies to predict buffalo BW, incorporating HW measurement. In these new studies, it would be crucial to develop equations considering the age and physiological stage of the animals, aiming to enhance predictions.
In conclusion, the quadratic model utilizing hip width as a predictor variable proves to be a viable method for estimating body weight with satisfactory precision in dairy buffaloes.
