Carcinoid tumours are rare, slow-growing tumours, arising from the enterochromaffin (Kulchitsky) cells disseminated throughout the gastrointestinal tract and bronchopulmonary system. Since 1980, the year of the first World Health Organization (WHO) classification of endocrine tumours, they have been classified as neuroendocrine tumours (NETs) [Reference Bosman1].

These tumours are described more frequently in the bronchi and gastrointestinal tract, but can also occur at other sites, such as pancreas, thymus and ovaries. Although appendiceal NETs are rare in children and adolescents, they are nevertheless the most common gastrointestinal epithelial tumours in this age group and are usually an incidental finding after a laparotomy or laparoscopy for appendectomy. Several series have reported cases in children, but the precise incidence in relation to the total number of appendectomies is not available. The general incidence ranges between 1: 100 000 and 1·14:1 million children per year [Reference Virgone2].

In Italy, 113 appendiceal NETs in children have been registered in the TREP project database from January 2000 to May 2013. The TREP (Tumori Rari in Età Pediatrica = Rare Tumours in Childhood) project is a multi-institutional network dedicated to very rare tumours in children and adolescents [Reference Virgone2, Reference Ferrari3]. Among the patients registered, in five cases the tumour was associated with parasitic bowel infection, being Schistosoma haematobium in one case and Enterobius vermicularis in four cases.

A 15-year-old boy was admitted to the emergency department with abdominal pain, vomiting and fever. He had been living in Togo up to 2 months before admission. Blood tests and ultrasound (US) of the abdomen led to a diagnosis of acute appendicitis: the US also showed thickening of the bladder wall with a pseudo-polyp image. The boy underwent an open appendectomy from which he recovered uneventfully. In the immediate post-operative days he presented persistent haematuria, and it was established that this symptom had been present for the past 2 years, but no diagnostic investigation or treatment had been performed. As a urinary infection was suspected, another US scan of the bladder was performed together with parasite tests on urine samples. The urine tests confirmed the diagnosis of schistosomiasis and the patient was treated with oral praziquantel resulting in prompt resolution of the infection. The pathological report demonstrated a well differentiated NET (maximum diameter 5 mm), in the tip of the appendix, confined to the appendiceal wall, with worms and eggs of S. haematobium in the lumen. The diagnostic work-up for appendiceal NET, that includes Octreoscan, abdomen US, urinary 5-hydroxyindoleacetic acid (5-HIIA) and serum serotonin, was negative and the boy remains free from disease 40 months after diagnosis (Table 1).

Table 1.

Clinical and pathological features

CR, Complete remission; mo., month.

* According to WHO classification, 2010 [Reference Bosman1].

† Other than appendicitis-related symptoms.

Four girls, with a mean age of 10·5 years (range 7–13·5 years), with the clinical suspicion of appendicitis (fever, abdominal pain, emesis) underwent an emergency appendectomy. In all four cases, pathology reports showed a small NET associated with the presence of E. vermicularis eggs in the lumen of the appendix. All tumours were well differentiated, limited to the appendiceal wall and small in size (Table 1). The post-operative investigations for appendiceal NET (1/4 Octreoscan, 4/4 abdominal US, 4/4 urinary 5-HIAA +/− serum chromogranin or serum serotonin) resulted negative and all patients are alive and free from disease at a median of 35·5 months after diagnosis.

Schistosomiasis (or bilharziasis) is a tropical infection due to different trematodes belonging to the Schistosoma genus. The main schistosomes infecting humans are: S. mansoni, which causes intestinal and hepatic schistosomiasis in Africa, the Arabian peninsula and South America; S. haematobium, causing urinary schistosomiasis in Africa and the Arabian peninsula; and S. japonicum, causing intestinal and hepatosplenic schistosomiasis in China, Philippines, and Indonesia.

Appendicitis caused by Schistosoma spp. infection is a well recognized entity but rarely observed in Western countries. In the case of the 15-year-old boy presented here, the urinary schistosomiasis had been present for at least 2 years during the patient's time in Togo, and no diagnostic investigation or treatment had been performed until he came to our attention due to symptoms of acute appendicitis. The patient had never complained of abdominal discomfort previously, but it is known that in some cases, S. haematobium infection of the intestinal tract remain asymptomatic.

The International Agency for Research on Cancer (IARC) considers S. haematobium as a group 1 carcinogen (carcinogenic to humans) responsible for helminth-induced human cancer, and S. japonicum as a group 2b carcinogen (possibly carcinogenic to humans) [4]. Besides bladder cancer, in which schiostosomiasis has a well-defined and proven carcinogenic role, other malignancies have been reported in association with schistosomiasis: these include colorectal carcinoma, lymphomas, hepatocellular carcinomas, rectal NETs and others [Reference Zanger5].

Only four cases of appendiceal NETs associated with schistosomiasis have been reported so far [Reference Maculotti and Pradella6–Reference Jiang8], but none of these were in childhood.

The following sequence of events in schistosomiasis-induced carcinogenesis has been suggested: chronic infection leads to Schistosoma eggs becoming trapped in the organ wall; the constant irritation and subsequent inflammation associated with the secretion of particular parasite factors may determine carcinogenic initiation and progression [Reference Abdel, Hassan and El-Sewedy9]. The oncogenic potential of S. haematobium has been further investigated: nude mice exposed to Chinese hamster ovarian cells treated with S. haematobium antigens developed sarcomas, and normal epithelial cells treated with S. haematobium total antigen showed an increase in proliferation, migration, invasion and decreased apoptosis, with high-level expression of Bcl-2 [Reference Botelho10]. It has been hypothesized that an oestradiol-related molecule could be responsible for the carcinogenic effects, binding host DNA bases and acting as an endogenous initiator [Reference Botelho, Machado and da Costa11] in a recent study, Jiang and colleagues [Reference Jiang8] demonstrated a higher expression of chromogranin A and Ki-67 in a series of three appendiceal goblet cell carcinoid tumours associated with schistosomiasis, indicating an increased cell proliferation and a neuroendocrine differentiation in mucosal crypt epithelium. These authors suggested that chronic schistosomal infection may contribute to the onset of goblet cell carcinoid tumours [Reference Jiang8].

E. vermicularis is a nematode, also known as pinworm, the most common pathogen among helminths. It is transmitted via the faecal–oral route and affects 2–28% of children worldwide. The disease, secondary to E. vermicularis, is innocuous, with egg deposition causing perineal, perianal, and vaginal irritation; in the intestine the worms lie primarily in the caecum.

Although rare, appendicitis due to oxyurasis infection has already been extensively described in the literature, but conversely E. vermicularis has not been linked to the onset of neoplastic disease: only two cases of concomitant appendiceal NETs and enterobiasis have been reported [Reference Andersson and Bergdahl12]. A cause–effect relationship between the co-existence of appendiceal NETs and E. vermicularis cannot be established, due to lack of biological and epidemiological data. However, some questions should be raised from these findings. Considering that the respective frequency rate of enterobiasis-related appendicitis and appendiceal NETs are both low in Western countries [Reference Arca13], is their concomitant manifestation only a coincidence? The rate of parasite-related appendicitis in the TREP database is 4·4%, slightly higher than that described in literature in Western countries (0·1–1·5%); however, on the other hand, paediatric E. vermicularis infections are very common and the true rate of parasite-related appendicitis may have been overlooked in the past, both by pathologists and surgeons. Moreover, it is still unclear whether appendiceal NETs themselves could be a cause of appendicitis and appendiceal infestation by E. vermicularis, in these cases it may only be an incidental finding: this may add confusion to the situation considering the scarcity of cases reported in the literature, as in the case series presented here which is extremely rare.

Nevertheless, for E. vermicularis one might cautiously hypothesize a carcinogenic process triggered by chronic local inflammation caused by parasites themselves or their products, as seen in helminth- and trematode-related carcinogenesis [Reference Vennervald and Polman14, Reference Coussens and Werb15]: the small size and low grading of all four tumours in our series could be in keeping with a hypothetical inflammation-based carcinogenesis.

We have described five cases of NETs and concomitant appendiceal parasitic infections. Although only a few similar cases are described in literature and currently there is no proven causal link between S. haematobium and NETs of the gastrointestinal tract, the carcinogenic effect of S. haematobium is well known, and general evidence on the biochemical basis of tumour induction has been described recently. By contrast, the carcinogenic potential of E. vermicularis infection is less clear. This is the first report in the English language which theorizes a possible carcinogenic effect of oxyurasis; however, to further confirm this hypothesis, more data, with a deeper analysis of larger series both in children and in adults, are needed.