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Two species of Carbacanthographis from India

Published online by Cambridge University Press:  03 June 2010

Bharati O. SHARMA ,
Urmila MAKHIJA  and
Pradnya KHADILKAR
Bharati O. SHARMA
Affiliation:
Agharkar Research Institute, G.G. Agarkar Road, Pune—411004, India.
Urmila MAKHIJA*
Affiliation:
Agharkar Research Institute, G.G. Agarkar Road, Pune—411004, India.
Pradnya KHADILKAR
Affiliation:
Agharkar Research Institute, G.G. Agarkar Road, Pune—411004, India.
*
Email: uv_makhija@hotmail.com
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Abstract

Two species of the lichen genus Carbacanthographis, namely C. marcescens and a new species, C. sorediata, are reported from India. The new species is characterized by a sorediate thallus, laterally carbonized exciple, submuriform ascospores, and salazinic and consalazinic acids in the thallus. A worldwide key to the species of Carbacanthographis is provided.

Keywords

Graphidaceaelichenized Ascomycotataxonomy

Information

Type
Research Article
Information
The Lichenologist , Volume 42 , Issue 4 , July 2010 , pp. 391 - 395
Copyright
Copyright © British Lichen Society 2010

Introduction

The lichen genus Carbacanthographis Staiger & Kalb was established by Staiger & Kalb (2002). It comprises species in the family Graphidaceae characterized by a convergent, carbonized exciple, labia covered with a distinct white pruinose layer in the upper part, warty periphysoids, and I− or weakly I+, muriform or trans-septate ascospores. So far the genus is known to include 17 species world-wide (Staiger Reference Staiger2002; Kalb Reference Kalb2004; Archer Reference Archer2006, Reference Archer2007; Lücking et al. Reference Lücking, Archer and Aptroot2009) and has been reported from the Neotropics, Africa, Australia, Singapore, Japan and the Solomon Islands. Vegetative propagules are generally rare in Graphidaceae, but in our recent studies in the family, we have discovered one sorediate species which belongs to the genus Carbacanthographis. This species is formally described in the present paper. A key to the species of Carbacanthographis so far known is also provided.

Materials and Methods

The study is based on herbarium specimens housed at the Ajrekar Mycological Herbarium (AMH). Sections of thalli and ascomata were mounted in water, 10% KOH (K), Lugol's solution (I), and lactophenol cotton-blue (LPCB). All measurements were made on material mounted in water. Secondary products were identified by thin-layer chromatography using standard methods (Culberson & Kristinsson Reference Culberson and Kristinsson1970; Culberson Reference Culberson1972; White & James Reference White and James1985) with the solvent systems toluene-dioxane-acetic acid (180:45:5) and toluene-ethyl acetate-formic acid (139:83:8). The plates were examined under UV light (365 nm).

The Species

Carbacanthographis marcescens (Fée) Staiger & Kalb

Biblioth. Lichenol. 85: 109 (2002).—Graphis marcescens Fée, Essai Crypt. Exot. (Paris) 1: 38 (1825).

(Fig. 1)

Fig. 1.

Carbacanthographis marcescens, habitus (76.584—AMH). Scale = 1mm

Fig. 2.

Carbacanthographis sorediata (holotype, AMH). A–C, habitus; D, vertical section of ascocarp and soralium; E, warty periphysoids; F, ascospores. Scales: A–C = 1mm; D = 50μm, E–F = 10 μm.

Remarks. Carbacanthographis marcescens has earlier been recorded from India as Graphina marcescens Fée (Patwardhan & Kulkarni Reference Patwardhan and Kulkarni1976; Makhija et al. Reference Makhija, Adawadkar and Patwardhan1992) and can easily be identified by its laterally carbonized exciple, warty periphysoids, powdery, white thalline cover, small muriform ascospores, and by the presence of salazinic, protocetraric and norstictic acids in the thallus. The species is widely distributed and occurs in Australia, Brazil, Cameroon, Cuba, Guyana, India, Solomon Islands, Thailand, and the USA. In India it has been collected in the evergreen forests of Kerala and Tamil Nadu situated in the Western Ghats and in the tropical evergreen rain forest of Andaman Islands. Corticolous pyrenocarpous and graphidaceous lichens and the thelotremes predominate in the lichen biota of the Andaman Islands.

Specimens examined (all AMH). India: Andaman Islands: South Andaman, Tarmugli Island, near Wandoor, 1985, P. K. Sethy & P. G. Patwardhan 85.1945, 85.1946; Redskin Islands, Wandoor, 1985, P. K. Sethy & M. B. Nagarkar 85.1991, 85.1992; ibid., P. G. Patwardhan & M. B. Nagarkar 85.1999, 85.2000, 85.2002, 85.2006, 85.2007. Kerala: Munnar-Kodai Road, below Yellapattii, 1976, P. G. Patwardhan & M. B. Nagarkar 76.584; Cardamom hills, Devicolam-Kumily Road, 17 km from Devicolam, 1976, P. G. Patwardhan & A. V. Prabhu 76.695; Thekadi, near Periyar house, 1976, P. G. Patwardhan & M. B. Nagarkar, 76.949. Tamil Nadu: Kodaikanal, Silver Caskade, 1975, P. G. Patwardhan & A. V. Prabhu 75.60, 75.406, 75.420, 75.421; Kodailkanal, Kodai-Munnar Road, 10 km to Berijam lake, 1975, P. G. Patwardhan & A. V. Prabhu 75.128, 75.160; Kodaikanal, near Golf Club, 1975, P. G. Patwardhan & A. V. Prabhu 75. 189; Dastoor Villa, Kodai lake, 1975, P. G. Patwardhan & A. V. Prabhu 75.330, near Daisy Bank, 1975, P. G. Patwardhan & A. V. Prabhu 75.356.

Carbacanthographis sorediata B. O. Sharma, Makhija & Khadilkar sp. nov

Similis Carbacanthographide marcescente sed thallo sorediato, ascosporis majoribus et acido consalazinico differt.

Typus: India, Tamil Nadu, Agasthyamalai (Agasthi hills), Upper Kodayar, 24 January 1983, P. G. Patwardhan & P. K. Sethy 83.250 (AMH—holotypus)

(Fig. 2A– F)

Thallus crustose, corticolous, epiphloeodal, greenish grey, finely cracked, with a thin pseudocortex, sorediate; soralia whitish, granular, with the photobiont layer being pushed upwards by vertical hyphae in the thallus (Fig. 2D).

Ascocarps lirellate, conspicuous, light pinkish to creamy, emergent, undulate, with conspicuous powdery pruinose cover, simple to branched, curved, up to 4 mm long, ends acute or round. Disc slit-like, covered laterally with light pinkish pruina and coated with warty periphysoids. Proper exciple convergent, laterally carbonized, base brown. Epithecium absent. Hymenium hyaline, not inspersed, I−, 60–100 μm high. Paraphyses simple. Periphysoids short, distinctly warty. Asci 8-spored. Ascospores hyaline, fusiform, submuriform, with 7–8 transverse and 1–2 longitudinal septa per segment, 17·5–27·5 × 7·5–10 μm, I+ colour reaction weak.

Fig. 3.

Distribution of Carbacanthographis species in India; ● C. marcescens, ■ C. sorediata.

Chemistry. Salazinic and consalazinic acids present.

Remarks. Carbacanthographis sorediata stands distinct amongst all the known species of Carbacanthographis in having a sorediate thallus with a unique type of soralia: in sections it appears as if the photobiont layer is pushed upwards by vertical hyphae in the thallus, to eventually form the soralia. Carbacanthographis marcescens can be differentiated in lacking soralia and having smaller ascospores of 12–17 × 5–7 μm, and a slightly different chemistry. Carbacanthographis amicta (Nyl.) Staiger & Kalb and C. salazinica also contain salazinic acid, but differ from the new species in having a thallus without soredia and a completely carbonized exciple.

The new species Carbacanthographis sorediata is confined to the Western Ghats (Fig. 3), which form a practically unbroken relief dominating the western coast of the Indian peninsula for almost 1600 km. From a botanical point of view, the Western Ghats occupy a special position on the Indian subcontinent for their interesting flora and are one of the richest lichen sites in India. The species was collected in the Agasthyamalai (Agasthi hills) in tropical moist evergreen montane forests at an elevation of c. 1800 m.

Additional specimens examined. India: Tamil Nadu: Agasthyamalai (Agasthi hills), Upper Kodayar, 1983, P. G. Patwardhan & P. K. Sethy 83.249, 83.356, 83.357 (AMH).

World-wide key to the species of Carbacanthographis

  1. 1 Thallus isidiate or sorediate ... 2

    Thallus neither isidiate nor sorediate ... 3

  2. 2(1) Thallus isidiate; asci (4–) 6–8-spored; protocetraric acid present; Australia ... ... C. hertelii Kalb & Staiger

    Thallus sorediate; asci 8–spored; salazinic and consalazinic acid present; India ... ... C. sorediata B. O. Sharma et al.

  3. 3(1) Excipular base not carbonized ... 4

    Excipular base carbonized ... 8

  4. 4(3) Ascospores trans-septate ... 5

    Ascospores muriform ... 6

  5. 5(4) Norstictic acid present; Vietnam ... C. induta (Müll. Arg) Lücking

    Stictic acid present; Japan ... ... C. iriomotensis (M. Nakan.) M. Nakan. & Kashiw.

  6. 6(4) Salazinic acid (major), protocetraric and norstictic acid (trace) present; ascospores 12–17 × 5–7 μm; Australia, Brazil, Cuba, Dominica and India ... ... C. marcescens (Fée) Staiger & Kalb

    Stictic acid present ... 7

  7. 7(6) Asci 1-spored; ascospores c. 100 μm long ... C. cleitops (Fée) Lücking

    Asci 6–8-spored; ascospores 47–75 × 18–30 μm; Cuba, Dominica ... ... C. triphoroides (M. Wirth & Hale) Lücking

  8. 8(3) Ascospores trans-septate ... 9

    Ascospores muriform ... 12

  9. 9(8) Ascospores 4 locular; asci 8-spored; ascospores 18–20 × 8–10 μm; psoromic acid present; Solomon Islands ... C. alloafzelii (A.W. Archer) A.W. Archer

    Ascospores more than 4 locular ... 10

  10. 10(9) Ascospores large, more than 50 μm long; asci 4–8–spored; ascospores 55–100 × 7–9 μm; protocetraric and lichexanthone present; Brazil, Paraguay ... ... C. candidata (Nyl.) Staiger & Kalb

    Ascospores small, less than 50 μm long ... 11

  11. 11(10) Ascospores 23–35 × 6–7 μm in size; stictic, constictic, menegazzic and hypostictic acids present; Brazil ... C. stictica Staiger & Kalb

    Ascospores 20–25 × 6–8 μm; protocetraric and lichexanthone acids present; Brazil ... C. chionophora (Redinger) Staiger & Kalb

  12. 12(8) Lichen substances absent; asci 8–spored; ascospores 20–40 μm long; Brazil ... ... C. coccospora (Aptroot) Aptroot & Lücking

    Lichen substances present ... 13

  13. 13(12) Ascospores more than 50 μm long ... 14

    Ascospores less than 50 μm long ... 15

  14. 14(13) Asci 8–spored; ascospores 55–90 × 11–18 μm; protocetraric acid and lichexanthone present; Brazil ... C. subalbotecta Staiger & Kalb

    Asci 1–spored; ascospores 120–180 × 25–30 μm; stictic, constictic and cryptostictic acids present; Brazil ... C. crassa (Müll. Arg.) Staiger & Kalb

  15. 15(13) Salazinic acid present ... 16

    Salazinic acid absent ... 17

  16. 16(15) Ascospores 14–18 × 6–7 μm; only salazinic acid present; New Pacific and Japan ... C. amicta (Nyl.) Staiger & Kalb

    Ascospores 19–23 × 7–8 μm; salazinic (major), consalazinic, norstictic and protocetraric (trace) acids present; Australia, Indonesia ... ... C. salazinica (A.W. Archer) A.W. Archer

  17. 17(15) Ascospores 22–26 × 10–12 μm; subpsoromic acid present; Solomon Islands ... ... C. hilli (A.W. Archer) A.W. Archer

    Ascospores 14–18 × 6–7 μm; protocetraric acid present; South America ... ... C. inspersa Staiger

We are grateful to the Department of Science and Technology, Government of India, New Delhi, for their financial support.

References

Archer, A. W. (2006) The lichen family Graphidaceae in Australia. Bibliotheca Lichenologica 94: 1–191.Google Scholar
Archer, A. W. (2007) Key and checklist for the lichen family Graphidaceae (lichenized Ascomycota) in the Solomon Islands. Systematics & Biodiversity 5: 9–22.Google Scholar
Culberson, C. F., Kristinsson, H. (1970) A standardized method for the identification of lichen products. Journal of Chromatography 46: 85–93.CrossRefGoogle Scholar
Culberson, C. F. (1972) Improved conditions and new data for the identification of lichen products by a standardized thin-layer chromatographic method. Journal of Chromatography 72: 113–125.CrossRefGoogle ScholarPubMed
Kalb, K. (2004) New or otherwise interesting lichens II. Bibliotheca Lichenologica 88: 301–329.Google Scholar
Lücking, R., Archer, A. W. & Aptroot, A. (2009) A world-wide key to the genus Graphis (Ostropales: Graphidaceae) Lichenologist 41: 363–452.CrossRefGoogle Scholar
Makhija, U., Adawadkar, B. & Patwardhan, P. G. (1992) Materials for a lichen flora of the Andaman Islands: VIII-The genus Graphina. Biovigyanam 18: 21–32.Google Scholar
Patwardhan, P. G. & Kulkarni, C. R. (1976) Some additions to the lichen flora of India IV: Graphis and Graphina (family Graphidaceae) Biovigyanam 2: 123–132.Google Scholar
Staiger, B. (2002) Die Flechtenfamilie Graphidaceae: Studien in Richtung einer natürlicheren Gliederung. Bibliotheca Lichenologica 85: 1–526.Google Scholar
White, F. J. & James, P. W. (1985) A new guide to microchemical techniques for the identification of lichen substances. British Lichen Society Bulletin 57 (Suppl.): 1–41.Google Scholar
Figure 0

Fig. 1. Carbacanthographis marcescens, habitus (76.584—AMH). Scale = 1mm

Figure 1

Fig. 2. Carbacanthographis sorediata (holotype, AMH). A–C, habitus; D, vertical section of ascocarp and soralium; E, warty periphysoids; F, ascospores. Scales: A–C = 1mm; D = 50μm, E–F = 10 μm.

Figure 2

Fig. 3. Distribution of Carbacanthographis species in India; ● C. marcescens, ■ C. sorediata.