This is the third edition of a book first published in 1996. It is interesting to note that the basic techniques of plant tissue culture have changed very little, but some of the applications have diversified in this time. The book is aimed at students, and as such contains some valuable material and well-described practical exercises. The section on the set-up of tissue culture laboratory is useful and the following chapters describe exercises in all the accepted aspects of plant tissue culture, concluding with a chapter on Agrobacterium-mediated transformation. The chapter contents are rather uneven however, and there is no real need in a slim volume aimed at student practical exercises for a lengthy review on plant tissue culture history with 200 references cited. The chapter outlines at the start of each chapter are simple and informative and clear headings make the chapters easy to follow. However, some chapters have questions embedded and some do not, and this creates an inconsistent tone. The Appendices on Useful Measurements and Solution Preparation would be of some value to a beginner, and the Glossary gives clear explanations of commonly used terms. The real problem with this volume is that, while informative and easy to read, it offers little new to an already very well-documented branch of plant science.