Dietary fibres, including psyllium and inulin, are non-digestible carbohydrates found in fruits, vegetables and legumes(1). An optimal intake of dietary fibre decreases the risk of type 2 diabetes, colon cancer and cardiovascular disease(2), but only 5% of the UK adult population consumes the recommended dietary fibre intake of 30 g/d(3). Optimal fibre intake is also thought to be important for patients. There is evidence from murine models that dietary fibre supplementation can improve tumour response and reduce normal tissue side effects caused by radiation in cancer patients(4). Studying the effect of dietary fibres on healthy older people in detail is the first step to designing clinical trials in cancer patients.
A within-subject, double-blinded study (n=40) was conducted on healthy older adults, over 60 years, to explore the response of the gut microbiota composition and activity to dietary fibre supplementation. Maltodextrin was given as placebo, with psyllium+inulin and inulin provided as diet interventions to be consumed for two weeks each, with a two-week washout period between treatments. Faecal samples for rRNA 16S sequencing, IgA and calprotectin analysis, as well as gut symptom and quality of life (QOL) questionnaires, were collected at the start and end of each intervention. The study was approved by the Human Studies Management Committee and Rowett Ethics committee.
Participants self-assessed QoL remained stable during the study (Anova, p>0.05). However, all interventions reportedly caused flatulence (Friedman, p<0.05), with psyllium+inulin supplementation slightly increasing early satiation (p=0.04) and rumbling (p=0.02) when compared to maltodextrin. Participants average fibre intake was 23.8 g/day (SD=8.0) at baseline, and remained consistent during the study (Anova, p=0.0525). IgA showed no significant change between maltodextrin, psyllium+inulin and inulin supplementation (2431±5490 µg/ml, 2024±2633 µg/ml and 1614±1683 µg/ml, Anova, p=0.906). Calprotectin levels showed no significant change between maltodextrin, psyllium+inulin and inulin intake (41.30±56.13 mg/kg vs 28.06±32.29 mg/kg vs 27.60±33.97 mg/kg, Anova, p=0.529). However, the intake of psyllium+inulin significantly increased propionate concentration (3.17±1.68 MM vs 3.99±2.15 MM, t-test, p=0.023), and inulin supplementation significantly increased acetate (12.52±4.69 MM vs 14.45±5.58 MM, t-test, p=0.01) and propionate (3.27±1.38 MM vs 3.88±2.05 MM, t-test, p=0.007) concentrations. Metastats and LEfSE showed an increase in the genus Anaerostipes after psyllium+inulin supplementation, and an increase in the genera Anaerostipes and Bifidobacterium after inulin supplementation.
Overall, the supplements were well tolerated, with no change in gut health markers IgA and calprotectin. Bifidobacteria are associated with increased apoptosis in cancer cells and reduced radiation-induced diarrhoea(5), whereas lower abundance of Anaerostipes are reported in colorectal cancer patients(6). As such, modifying the gut microbiota using dietary fibres to increase the abundance of Bifidobacteria and Anaerostipes could advance patient treatment.