HPLC instrument performance metrics and validation

doi:10.1017/CBO9780511732263.025

Appendix B - HPLC instrument performance metrics and validation

Published online by Cambridge University Press: 05 March 2012

Aimee R. Neeley,

Crystal S. Thomas,

Stanford B. Hooker and

Laurie Van Heukelem

Edited by

Suzanne Roy,

Carole A. Llewellyn,

Einar Skarstad Egeland and

Geir Johnsen

Show author details

Suzanne Roy: Affiliation:
Université du Québec à Rimouski, Canada
Carole A. Llewellyn: Affiliation:
Plymouth Marine Laboratory
Einar Skarstad Egeland: Affiliation:
University of Nordland, Norway
Geir Johnsen: Affiliation:
Norwegian University of Science and Technology, Trondheim

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Summary

Currently, there are over 90 companies that offer HPLC hardware and accessories, and more than 30 that offer complete systems. Given the myriad choices available in the marketplace, the discerning chromatographer needs to approach equipment purchases with a critical mindset and a clear understanding of what they require from an HPLC system or component. This appendix covers some of the features available in HPLC autosamplers, pumps, detectors and ovens. It is not meant to be a definitive catalog of available HPLC hardware components and design elements. Instead, it is designed to call attention to some of the features available in specific HPLC hardware that the authors of this appendix have researched in the context of how these decisions can affect one's ability to produce consistent, high quality pigment results. A thorough review of the basics and advancements in HPLC hardware is covered in the third edition of Introduction to Modern Liquid Chromatography (Snyder et al., 2010).

To make informed decisions regarding one's needs in HPLC hardware, one must understand the component design (and software control thereof) from the perspective of its contribution to combined uncertainty. Uncertainties in pigment results related to hardware characteristics are most often associated with injectors and detectors, and to a lesser extent, column oven design and pump capabilities. The uncertainties of the latter are often related to implementation of a method (e.g. baseline disturbance).

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Type: Chapter
Information: Phytoplankton Pigments
Characterization, Chemotaxonomy and Applications in Oceanography
, pp. 636 - 649
DOI: https://doi.org/10.1017/CBO9780511732263.025 [Opens in a new window]
Publisher: Cambridge University Press
Print publication year: 2011

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