Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1Starting a New Laboratory and Training Protocols
- Chapter 1Establishing and Equipping a New IVF Laboratory
- Chapter 2Basic Embryology Skills and Responsibilities in the IVF Laboratory
- Chapter 3Sperm Preparation for IVF
- Chapter 4Oocyte Pick-Up for IVF
- Chapter 5Embryologist Training: Embryo Transfer
- Chapter 6ICSI for IVF
- Chapter 7Embryo Assisted Hatching for IVF
- Chapter 8Embryo Biopsy for IVF
- Chapter 9Vitrification for IVF
- Section 2Pre-procedure Protocols
- Section 3Gametes
- Section 4Insemination/ICSI
- Section 5Fertilization Assessment
- Section 6Embryo Assessment: Morphology and Beyond
- Section 7Embryo Cryopreservation
- Section 8Embryo Transfer
- Section 9Quality Management
- Index
- References
Chapter 8 - Embryo Biopsy for IVF
Training Protocol
from Section 1 - Starting a New Laboratory and Training Protocols
Published online by Cambridge University Press: 07 August 2023
Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1Starting a New Laboratory and Training Protocols
- Chapter 1Establishing and Equipping a New IVF Laboratory
- Chapter 2Basic Embryology Skills and Responsibilities in the IVF Laboratory
- Chapter 3Sperm Preparation for IVF
- Chapter 4Oocyte Pick-Up for IVF
- Chapter 5Embryologist Training: Embryo Transfer
- Chapter 6ICSI for IVF
- Chapter 7Embryo Assisted Hatching for IVF
- Chapter 8Embryo Biopsy for IVF
- Chapter 9Vitrification for IVF
- Section 2Pre-procedure Protocols
- Section 3Gametes
- Section 4Insemination/ICSI
- Section 5Fertilization Assessment
- Section 6Embryo Assessment: Morphology and Beyond
- Section 7Embryo Cryopreservation
- Section 8Embryo Transfer
- Section 9Quality Management
- Index
- References
Summary
Pre-implantation genetic testing for aneuploidies (PGT-A) of embryos involves confirming the chromosomal status of the embryo through assessment of biopsied cells. Most miscarriages and failed implantations are believed to be due to abnormal chromosomal status. Possessing the correct chromosome complement increases the ability of an embryo to implant and result in a live birth. Historically, biopsies occurred at the cleavage stage. However, with more cells being able to be biopsied at the blastocyst stage, representing a smaller proportion of the embryo, blastocyst biopsy is currently regarded as a safer procedure with increased sensitivity for detecting mosaics. Therefore, as the benefits of blastocyst biopsy became more evident, blastocyst biopsy has gradually become the method of choice in most in vitro fertilization (IVF) laboratories. As a technical procedure, lack of blastocyst biopsy skills has been an obstacle for some clinics to adopt this practice. This chapter outlines a structured methodology to train a biopsy practitioner to acquire the competency to confidently perform this procedure in a consistent manner that is safe for the embryos whilst maximizing the chance of pregnancy.
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- Principles of IVF Laboratory PracticeLaboratory Set-Up, Training and Daily Operation, pp. 54 - 64Publisher: Cambridge University PressPrint publication year: 2023
References
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