In recent years, reliable aseptic closed vitrification systems have been accepted instead of open devices and have changed the ART-cryopreservation landscape in the combat against any microbiological exposure. Still, misunderstanding on the balances in a vitrification method and inappropriate implementations expose IVF laboratories to inconsistent survival and pregnancy outcomes. The present set-up and training of a microdroplet vitrification model for human blastocysts takes into account some important quality-control factors to minimize technical variation with consistent good laboratory practice. A uniform approach of the physical environment at 37 °C was validated with a ready-to-use DMSO free vitrification kit plus Ssucrose warming kit and a closed storage device with a hole carrier, based on optimal visualization, simplicity of loading/recovery, consistent volumes and ionomeric-resin straws with secured seals. The microdroplet closed vitrification model offers safety, reliability, consistency with successful results towards benchmark survival / transfer rates (>95%) and promising day 5 vitrified blastocyst live birth rates (>30%).