Objectives/Goals: Sleep is a useful intervention target to improve mental and physical health. Yet, the contextual factors that impact sleep in real-life settings are poorly understood. We assessed how psychological and socioenvironmental factors influence self-reported sleep in a large, US sample (Study 1) and sleep physiology in a prospective cohort (Study 2). Methods/Study Population: Study 1: 13,483 participants (63% males, mean age 46, 24% non-White) completed on-demand blood pressure (BP), self-reported stress, physical health, and sleep duration assessments for 3 weeks. Study 2: 42 Black adults (23% males, mean age 42) completed up to six ambulatory sleep recordings at home for 2 weeks. Stage durations for rapid eye movement (REM) and non-REM Stages 1, 2, and 3 were determined. Daily self-reports of stress and physical health were also gathered. For both studies, residence zip codes were linked to a national database to quantify neighborhood factors (i.e., built environment, poverty, social cohesion, etc.). Multiple linear regressions and mixed-effect models assessed whether neighborhood factors, demographics, health indices, and stress could predict sleep durations. Results/Anticipated Results: In Study 1, neighborhood disadvantage, perceived stress, and systolic BP were associated with shorter sleep durations. Better self-reported health predicted longer sleep durations. Black, Native, Latino/as, and Pacific Islander participants had shorter sleep durations, whereas White participants had longer sleep durations. An interaction between perceived stress intensity and health indicated that better health (+1SD) protected sleep from increasing stress. Preliminary analyses in 26 participants from Study 2 revealed that neighborhood disadvantage and self-reported poor health are associated with shorter Stage 2 sleep durations. However, daily stress did not predict stage durations, and no interactions emerged. Discussion/Significance of Impact: Neighborhood disadvantage led to short sleep in two studies, with novel findings indicating that Stage 2 durations may be most sensitive to structural disadvantage. Interventions that invest in material resources to reduce neighborhood inequities may be an innovative approach to protect sleep and mitigate related illness.

Objectives/Goals: This research develops a Ruthenium (III)–Fruquintinib complex for lung cancer treatment. Goals include structural characterization using MALDI-TOF and UV–Vis spectroscopy, synthesis optimization, and antitumor activity assessment comparing efficacy against Fruquintinib alone through cell studies. Methods/Study Population: The Ruthenium(III)–Fruquintinib complex is synthesized through an optimized coordination chemistry protocol ensuring reproducibility. Small-scale reactions defined baseline conditions, monitored by UV–Vis spectroscopy for ligand–metal charge transfer. After successful crystallization, the method was scaled up consistently. Structural characterization using MALDI-TOF mass spectrometry will be performed in collaboration with the MSRC, University of Puerto Rico, with infrared spectroscopy as a complementary validation. Biological evaluation will use NSCLC cell lines to compare cytotoxic efficacy against Fruquintinib alone, employing MTT assays to determine IC50 and potential synergistic effects. Results/Anticipated Results: Lung cancer remains the leading cause of cancer-related mortality, with 125,070 projected deaths in 2024. Current treatments for metastatic disease include surgery, chemotherapy, radiation, and targeted therapy. Fruquintinib (FQ), a VEGFR inhibitor, showed promise in early NSCLC trials but limited efficacy in advanced stages. Ruthenium (III) complexes, based on the hard–soft acid–base principle, offer potential anticancer properties. Combining FQ with Ruthenium (III) may enhance therapeutic outcomes. Preliminary synthesis yielded reproducible crystals confirmed by UV–Vis signals. Future MALDI-TOF characterization and biological assays will assess structural validity and antitumor potential. Discussion/Significance of Impact: The Ruthenium (III)–Fruquintinib complex could enhance Fruquintinib efficacy in advanced NSCLC. This approach demonstrates the potential of metal-based drug design to improve cancer therapies, offering insights into combinatorial strategies and advancing coordination chemistry in medicinal research.

Objectives/Goals: Rush University developed human-centered design studios to help translation research teams overcome recruitment and retention barriers in clinical trials. We describe the adaptation process and lessons learned when implementing these studios at The University of Chicago (UChicago). Methods/Study Population: UChicago studios followed the core Rush design, including study presentation, process mapping, partner identification, insight framing, and idea generation, but were adapted to local context and resources. The main adaptation transitioned the role of studio facilitator from a faculty human-centered design expert to trained research coordinators. This process included structured training and adaptation of workflows within the institutional context. Regular cross-institutional meetings provided a forum to debrief challenges and implement needed adjustments. Evaluation data included attendance records, post-session surveys, and regular debriefs to assess fidelity and adaptation. Results/Anticipated Results: In 2025, six 1-hour studios were held at UChicago, averaging eight participants per session (range 6–10; n=50). Participants included faculty and research, administrative, and community engagement staff. Feedback surveys (n=14) yielded a Net Promoter Score of 85 (12 promoters, 2 passives, and 0 detractors). Among respondents, 71% found sessions useful, 50% were first-time attendees, and 57% attended primarily to help research teams overcome recruitment barriers. Participants described the studios as valuable for generating concrete solutions and fostering cross-departmental connections. Key challenges included sustaining attendance, expanding presenter recruitment, and engaging more faculty. Discussion/Significance of Impact: Replication of design studios at UChicago demonstrates that the model can be sustained through trained non-faculty facilitators. Key lessons include the importance of faculty mentorship, facilitator training, consistent coordination, and cross-institutional learning to strengthen implementation.

Objectives/Goals: 1. Determine if adverse childhood experiences in our novel database are associated with adverse mental and physical health outcomes. 2. Assess differences in developmental and neuropsychological functioning in children with PME and/or opioid exposure. 3. Predict long-term impact of prenatal METH, opioid, and co-use exposures on cognitive development. Methods/Study Population: The Minnesota Foster Care and Adoption Population-level Database includes demographics, birth data (date of birth, gestational age, birth weight, birth length), medical visits and hospital admissions, growth measures over time (height, weight, BMI, and head circumference),vitals, labs, ICD diagnoses, type of prenatal substance exposure (PSE), ACEs (PEARL standards), caregiver placement types (adoption, traditional foster care, kinship care, institutional care, orphanage care, respite care), number of caregiver transitions, and risky behaviors (being detained, suicidal ideation or attempt, self-injurious behaviors, and substance use). Results/Anticipated Results: The study includes 2,725 participants, comprising both adopted children and those in various foster care settings. Transitions are frequent, with only 17% of participants experiencing no transitions. The most common numbers of transitions are two (10%), four (10%), five (9.8%), and six (9.7%). The leading adverse childhood experiences are household substance abuse, mental illness, physical neglect, domestic violence, and physical abuse. Alcohol, tobacco, and methamphetamine are the most frequently used substances during pregnancy, a trend that persists with polysubstance exposure. Adverse childhood experiences are linked to ADHD, conduct disorders, depression, anxiety, mood disorders, and adjustment disorders. Discussion/Significance of Impact: Our findings could help identify a comprehensive profile of developmental and neuropsychological functioning in PSE populations. Our strongest asset is our database, which controls environmental factors better than the general population when we study neurodevelopmental outcomes of PSE.

Objectives/Goals: The objective of this study is to quantify the physical properties of the human vitreous noninvasively in vivo, by applying Brillouin Spectroscopy. Our goal is to measure the Brillouin shift and linewidth of the human vitreous in normal aging, diabetes, and myopia. Methods/Study Population: Our study population will include three groups: patients with emmetropia and no diabetes, patients with diabetes without proliferative retinopathy, and patients with axial myopia. We will exclude patients with any prior vitreoretinal surgery or other systemic or ocular conditions that may affect the vitreous such as rhegmatogenous retinal detachment or systemic collagen disorders. We will measure baseline characteristics including age, axial eye length, diabetes duration, and A1c level. We will perform a complete dilated eye exam and obtain imaging including optical coherence tomography in addition to Brillouin microscopy of the anterior and mid vitreous. Results/Anticipated Results: We anticipate the following findings based on evidence from ex vivo studies which show a decrease in vitreous viscosity in the anteroposterior axis physiologically, in addition to a normal decrease in vitreous viscosity with age. In diabetes, we expect to find an increase in the viscosity of the vitreous proportional to the duration and severity of diabetes due to glycation of the vitreous. In myopia, we expect to find accelerated age-related decline of the viscosity of the vitreous, proportional to the degree of myopia present. Discussion/Significance of Impact: Aging, diabetes, and myopia are risk factors for the development of blinding conditions like diabetic retinopathy and rhegmatogenous retinal detachment. By quantifying the mechanical changes of the vitreous related to these risk factors, we hope to identify patients at high risk for vision loss for preventive treatment and monitoring.

Objectives/Goals: This study evaluated whether the Geriatric Acute and Post-acute Fall Prevention (GAPcare) intervention reduced fall-related emergency department (ED) visits and hospitalizations within 12 months of the index visit at three EDs and described adherence to pharmacy and physical therapy (PT) recommendations. Methods/Study Population: We enrolled ED patients aged ≥65 years presenting within 7 days of an accidental fall (excluding syncope, assault, or vehicle strike), who were community-dwelling, expected to be discharged, and without fractures preventing PT. We used the six-item screener for cognitive screening. Patients scoring ≤4 assented with proxy consent. Participants were randomized 1:1 to usual care or GAPcare intervention (ED-initiated pharmacy and PT consults). The primary outcome was fall-related ED visits within 12 months. Outcome assessors were blinded. Analyses followed intention-to-treat and adjusted for age, sex, prior falls, comorbidity index, activities of daily living, injury status, site, index ED visit, hospitalization, and assistive device use using negative binomial regression. Results/Anticipated Results: Among 196 patients, 100 received usual care and 96 received the GAPcare intervention. Median age was 78 (IQR 72–84), 68.4% were female, and 83.1% were white. Fall-related ED visits occurred in 30% of both groups (adjusted IRR 0.90, 95% CI 0.53–1.53). Fall-related hospitalizations were significantly lower in the intervention group (11% vs 21%; adjusted IRR 0.45, 95% CI 0.20–0.96), Adherence to pharmacy recommendations was 81%, and PT recommendations was 65%, with frequent medication optimization and assistive device use. Discussion/Significance of Impact: While GAPcare did not reduce fall-related ED visits, it significantly lowered fall-related hospitalizations and showed high adherence to fall prevention recommendations. These findings highlight GAPcare’s feasibility and potential to address fall risk during a critical window.

Objectives/Goals: Meaningful community engagement is critical to trustworthy translational research, yet engagement strategies outside structured meetings are rarely described. For a study of HIV PrEP implementation on college campuses, a Community Involved Board (CIB) participated in between-session activities (BSAs), described below. Methods/Study Population: Six CIB members were recruited to attend bi-monthly sessions from 2024 to 2025. The research team developed each BSA from relevant components of key theoretical frameworks including Labor Process Theory, Critical Pedagogy, and Community-Based Participatory Research. These frameworks informed the design of activities such as critical reflection journals, media audits of health messaging on public and social platforms, and synchronous activities such as CIB-led campus tours for researchers. BSA “responses” informed the direction of subsequent sessions. The five BSAs were optional, and participants were compensated $15–$25 per activity. Feedback from research team debriefing sessions and CIB member evaluations guided BSA refinement and implementation. Results/Anticipated Results: CIB members completed nearly all BSAs and perceived their input as meaningfully shaping session and project goals. Evaluations and debriefing notes revealed that BSAs promoted continuity in participation, individual and group agency, and reflexivity among CIB members. Researchers noted improved depth, specificity, and sharing during CIB sessions and increased communication between CIB members and the research team regarding logistics, successes, and challenges related to BSA completion. Reported barriers to BSA implementation included competing demands (from family, work, and school) and inconsistent expectations for BSA completion. The research team addressed challenges by offering timeline flexibility, altering compensation structures and reflexively examining and discussing power disparities. Discussion/Significance of Impact: Translational research relies on responsive and sustainable community engagement. The BSA approach demonstrates how intentional, theory-informed activities can translate engagement principles into practice, maintaining meaningful and authentic collaboration beyond structured community meetings.

Objectives/Goals: This proposed study aims to explore whether geographic patterns exist in the prevalence of anxiety diagnoses among adult patients (ages 18–64 years) using EHR data from three counties in North Carolina. Methods/Study Population: This proposed study will employ a retrospective, cross-sectional analysis of de-identified EHR data, examining anxiety diagnoses across geographic locations. To analyze the data, a multi-step approach will be used, combining geospatial analysis, descriptive statistics, and inferential statistical tests. Results/Anticipated Results: Findings may reveal geographic disparities in anxiety prevalence, highlighting potential social determinants of mental health and social risk factors. Identifying spatial patterns could inform targeted interventions to improve mental health equity and resource allocation in high-burden areas. Discussion/Significance of Impact: Understanding the geographic distribution of anxiety diagnoses can help guide policy and healthcare efforts to address mental health disparities. This study contributes to the growing body of research leveraging EHR data to examine population-level mental health trends.

Objectives/Goals: This study evaluates the impact of a pharmacist-delivered peer educational intervention on buprenorphine dispensing behavior for opioid use disorder (OUD) in Appalachian Kentucky. Methods/Study Population: Based on themes from focus group analyses of our prior work, we developed a pharmacist-delivered peer educational intervention consisting of brief, on-site academic detailing sessions (i.e., mini visits) and live continuing pharmacy education webinars. Trained academic detailing pharmacists conducted mini-visits to 113 community pharmacies across 23 counties (20 lowest and 3 of the next 5 lowest buprenorphine dispensing rates per individual with OUD), providing materials while webinars reinforced confidence and addressed stigma. Changes in pharmacists’ self-reported buprenorphine dispensing behaviors were evaluated using a validated pre-post survey offered to all pharmacists in each pharmacy. Analyses used unpaired t-tests for continuous scores variables and chi-square for categorical variables Results/Anticipated Results: A total of 128 of 131 participants (97.7%) completed the pre-survey and 95 of 131 (72.5%) completed the post-survey. Sex was similar between individuals in the pre- and post-surveys (50.0% vs 52.6% female, P=0.7). Most worked in independent pharmacies (64.8% pre, 67.4% post) and had ≥10 years in practice (63.3% pre, 68.4% post). Buprenorphine dispensing was reported by 85.9% pre and 88.3% post. After the intervention, participants showed greater willingness for telehealth prescriptions, with a mean change of +0.2 points (95% CI: 0.001–0.46, P = 0.0038) on a four-point scale. They also reported more welcoming environments toward individuals receiving buprenorphine treatment (five-point scale; mean change: +0.4, 95% CI: 0.05–0.8, P = 0.027). Discussion/Significance of Impact: Our pharmacist-delivered peer intervention enhanced pharmacists’ willingness to dispense buprenorphine via telehealth and fostered supportive pharmacy environments. Next, we will assess its effectiveness in expanding buprenorphine treatment access across Kentucky using county-level dispensing data.

Objectives/Goals: Post-stroke fatigue (PSF), marked by early exhaustion and reduced activity tolerance, affects over half of stroke survivors. This novel study used All of Us Research Program data to explore factors linked to PSF, addressing gaps left by prior studies with methodological limitations. Methods/Study Population: We conducted a cross-sectional analysis of ischemic stroke survivors from the All of Us Research Program Controlled Tier dataset. Participants were identified using ICD-10 diagnosis I63 for cerebral infarction, with strokes occurring prior to survey completion. Self-reported fatigue was assessed using a 5-point Likert scale (none to very severe). Demographics, pre-stroke comorbidities, and health status factors were extracted from electronic health records and surveys. Proportional odds regression models identified factors associated with higher fatigue levels. Results/Anticipated Results: 4,009 ischemic stroke survivors met inclusion criteria (mean age 64±13 years, 53% female, median 2 years post-stroke). Fatigue was documented in 56% of participants through electronic health records, while 84% reported some level of fatigue on surveys. In multivariable analysis, factors significantly associated with greater fatigue included younger age (OR: 0.98, 95% CI: 0.98–0.99, p<0.001), female sex (OR: 1.38, 95% CI: 1.21–1.57, p<0.001), current smoking (OR: 1.19, 95% CI: 1.05–1.36, p=0.008), diabetes mellitus (OR: 1.34, 95% CI: 1.1–-1.54, p<0.001), chronic obstructive pulmonary disease (OR: 1.37, 95% CI: 1.16–1.62, p<0.001), and mood disorders (OR: 1.96, 95% CI: 1.72–2.23, p<0.001). Discussion/Significance of Impact: This is among the first studies to examine PSF using large, representative datasets. With 84% reporting symptoms, findings reveal under recognition in clinical settings and identify modifiable risk factors to guide targeted, evidence-based interventions.

Objectives/Goals: Analyze risk factors among college students and their likelihood of experiencing or perpetrating sexual violence. Critique the process to develop a screening tool to match students to existing programming. Examine principles of implementation science in overcoming barriers to connect each student to the most appropriate prevention programming. Methods/Study Population: Research questions: What needs to be in a screening tool to gauge each incoming college student’s risk factors and likelihood for future sexual violence victimization and perpetration? How can the tool be designed using principles of implementation science with end-user feasibility in mind? For this protocol, I will initiate a participatory research process to guide the development of a screening tool for incoming college students in a mid-western sample of the USA to assess risk factors for sexual violence victimization and perpetration. I will assemble a Technical Expert Panel to develop the screening tool using existing standardized measures. I aim to recruit a diverse panel of researchers and practitioners in sexual violence prevention, including sexual assault survivors and peer educators. Results/Anticipated Results: The anticipated result will be a screening tool to predict the likelihood of victimization and perpetration for each incoming college student over one academic year. Next steps would be to pilot-test the screening tool to determine its effectiveness. The long-term goal would be to match each student to the most appropriate evidence-based prevention program based on individualized screening results. This could include existing evidence-based programs that prevent or decrease severity of victimization, revictimization, alcohol-facilitated victimization, perpetration, perpetration among high-risk students, and perpetration among fraternity members. Considering barriers and stakeholder engagement from the onset of the project aims to increase the feasibility of implementation in practice. Discussion/Significance of Impact: Quantifying an approximation of how many students would benefit from which types of programming can help colleges determine where to invest their time and resources to best prevent sexual violence. Guiding each incoming student to the most appropriate evidence-based prevention program could decrease rates of campus sexual violence.

Objectives/Goals: Our aim is to determine the pathogenesis of intramammary infection of H5N1. First, we must induce lactation, and our aim was to create a protocol to induce lactation without impregnating mice. We hypothesize that the immunological milieu of a lactating mammary gland is highly conducive for influenza viral infection. Methods/Study Population: Nine 10-week-old female mice of unidentified strain were placed in cohorts of lactating versus non-lactating versus control (undeveloped mammary glands) groups. Animals were co-housed within their group. Lactogenic hormones were used to induce mammary development and lactation, including estradiol, progesterone, oxytocin, and prolactin. Mice were inoculated with a comparative strain to H5N1, H1N1, due to its increased availability and safety standards. Immunopathology of mammary tissues and lungs will be assessed by histology, viral titration, and flow cytometry, and data analysis is pending. Results/Anticipated Results: All seven mice that were chosen to be injected with lactogenic hormones developed mammary tissue beyond the pubertal stage and into the reproductive stage, as expected if they were pregnant. Of those seven mice, the three that were given prolactin and oxytocin for milk production and let down were able to lactate. Plaque assays of mammary and lung tissue revealed no viral titers. Flow cytometry of innate surface markers showed varying results and were deemed inconclusive. Based on the preliminary data, it is inconclusive whether the mammary tissue was able to support H1N1 virus replication. Discussion/Significance of Impact: Better understanding of the immunopathology of the mammary gland and how it contributes to disease and disease transmission of influenza in mammalian species will be essential for the development of measures to control disease outbreak and minimize economic loss, especially in species such as cattle and goats.

Objectives/Goals: This study aimed to explore the feasibility of implementing sustainable depression screening services within an appointment-based pharmacy. Methods/Study Population: A qualitative observational study was conducted at a rural, appointment-based community pharmacy. Following staff consent, unstructured interviews and workflow observations were performed over a 1-week period to characterize existing processes and identify potential integration points for screening. Field notes from observations and interviews were supplemented with an anonymous Qualtrics survey assessing perceived barriers and facilitators to depression screening implementation. Results/Anticipated Results: The pharmacy filled approximately 200 prescriptions daily and employed one pharmacist and two to four bilingual technicians serving a predominantly Hispanic population. Both the pharmacist and staff were supportive of implementing depression screening and viewed it as compatible with their MedSync workflow. Reported barriers included the lack of a private counseling area and concerns regarding patient comfort discussing mental health due to stigma. Discussion/Significance of Impact: Appointment-based pharmacies are well suited to integrate depression screening into existing workflows. Future implementation efforts should address structural and stigma-related barriers to optimize feasibility and sustainability.

Objectives/Goals: This poster overviews the national landscape of policies related to patient engagement for research known as opt-in or opt-out policies. The evaluation compares policy types, changes to those policies, managing institutional hurdles, the resources used to support patient engagement for research, and shares best practices. Methods/Study Population: We identified peer academic medical institutions with active CTSA funding from public records and invited the contact PI of 37 institutions to speak about their patient engagement policies. We connected with 26 of these institutions in 30–60 minute discussions with members of CUIMC, WCM, and NYP. Each participant consented to being recorded for note-taking purposes before sharing an open-ended narrative about their patient engagement policies. Questions were completed throughout this process to address policy specifics, institutional resources, ethical considerations, and patient engagement. Results/Anticipated Results: In speaking with 26 peer institutions, we identified 50% have continuously operated under an opt-out policy with an additional 25% of institutions having previously switched from opt-in policies to opt-out. Those having undergone a policy switch commonly did so in alignment with an institutional implementation of an EHR system. The majority of opt-out institutions had special populations that were excluded from this policy for a multiple of ethical and practical considerations. Institutional decisions on policy type often centered on patient needs and experience, weighing patient autonomy versus patient privacy. Discussion/Significance of Impact: There has been a national shift toward opt-out patient engagement policies, frequently aligning with the launch of an EHR system. Those switching to opt-out policies often cited patient autonomy as their guiding ethical principle and chose to engage with patient advocacy groups following the policy implementation for operational optimization.

Objectives/Goals: 1. Explore how to increase shared leadership of CE Studio by leveraging the expertise of community facilitators. 2. Understand processes implemented to enhance power sharing between community and academic partners participating in CE Studios. Methods/Study Population: In partnership with a community co-investigator, community members (N= 35) were recruited to serve as facilitators for the Northeast Ohio CTSC Community Engagement (CE) Studios starting in October 2024. A four-phase innovation method was conducted to onboard community facilitators and solicit feedback to increase power sharing during our CE Studios – Phase 1: Developing Facilitator Pipeline, Phase 2: Engaged Training and Development, and Phase 3: Enhancing Expert Experience. A selection of facilitators participated in virtual or in-person CE Studio (N= 4) to pilot Phase 4: Team Integration. Together, these innovations enhanced community expert recruitment and shared leadership of the CE Studio while strengthening the value of the CE Studio for investigators seeking community input. Results/Anticipated Results: We anticipate enhanced CE Studio outcomes by implementing community facilitator guidance and innovation throughout the CE Studio framework. Discussion/Significance of Impact: Empowering facilitators to provide their expertise throughout the full scope of the CE Studio framework through coaching, expert recruitment, training, investigator preparation, and reporting enhances the CE Studio experience. This power sharing increases trust and quality of the CE Studio experience and enhances academic partnerships.

Objectives/Goals: Although diet quality is known to influence psychological health, this is under-explored in studies designed to improve diet quality. This secondary analysis aims to investigate the effects of culinary-focused nutrition education relative to standard nutrition education on dimensions of psychological health and well-being. Methods/Study Population: This is an exploratory analysis of the SPICE UP MyPlate Pilot Study, a randomized clinical trial investigating the effects of a novel 12-week culinary-focused nutrition education intervention on diet quality relative to standard nutrition education in healthy adults aged 31–59 years. The intervention included eight modules focused on flavorful healthy eating. The control group received a printed MyPlate resource about healthy eating. Psychological health and well-being outcomes included depression and anxiety symptoms, perceived stress, loneliness, and psychological well-being. Linear mixed models will be used for analysis. Results/Anticipated Results: Data collection for the SPICE UP MyPlate Pilot Study is ongoing at the time of this writing and will be completed in December 2025. All baseline visits have been completed, and 55 participants have been randomized. At baseline, the sample is predominately female (76% female; 24% male) and White (91% White; 5% Asian; 4% African American) with a mean age of 46 ± 8 years and a mean BMI of 27.2 ± 6.39 kg/m2. Results of this analysis are anticipated to show the preliminary effectiveness of culinary-focused nutrition education compared to standard nutrition education on 1) depression and anxiety symptoms, 2) perceived stress, 3) loneliness, and 4) psychological well-being. Discussion/Significance of Impact: Poor diet quality and poor psychological health are independent risk factors for leading causes of death. Understanding the effectiveness of culinary-focused nutrition education for improving diet quality and psychological health is important for developing population-level interventions that meaningfully reduce disease risk.

Objectives/Goals: We aim to develop new tissue-engineered ear tissue that more closely mimics native elastic cartilage and maintains its topographic form for reconstructive auricular surgery. By integrating decellularized cartilage (DCM) into chondrocyte-seeded collagen matrices, we seek to improve structural stability to support future clinical translation. Methods/Study Population: Bovine auricular chondrocytes were isolated via enzymatic digestion, expanded to passage 3, and encapsulated at 25 million cells/mL into four construct formulations composed of 1% type I collagen mixed with varying amounts of DCM: (1) 100% collagen, (2) 70% collagen + 30% DCM, (3) 40% collagen + 60% DCM, and (4) 10% collagen + 90% DCM. DCM was prepared by zesting ovine rib cartilage into ~0.5 mm³ flakes, followed by chemical and enzymatic decellularization. Constructs were packed into 3D-printed scaffolds and cultured for 0, 1, or 2 months. At each timepoint, constructs were assessed for gross volume, volume retention (normalized to baseline), and angle at the dome–base interface as a geometric surrogate for shape retention. Histology evaluated cell distribution and cartilage-specific matrix deposition. Results/Anticipated Results: At baseline, volumes were comparable across groups. By 1 and 2 months, constructs with higher DCM content yielded significantly greater volume retention. At 2 months, mean retention in the 90% DCM group was 94.0%±6.2%, compared to 64.7%±4.1% in 30% DCM (p<0.01) and 74.7%±5.9% in 0% DCM (p<0.05). Angle analysis likewise showed that higher DCM preserved sharper dome–base curvature. Mean angle change at 2 months was 3.0°±3.6° in 90% DCM versus 17.6°±5.7° (70% DCM, p<0.01), 16.3°±5.7° (30% DCM, p<0.05), and 21.6°±8.1° (0% DCM, p<0.001). In all groups, histology confirmed chondrocyte viability, and inclusion of DCM did not hinder cell distribution in collagen. These findings suggest DCM reinforces construct stability, and we anticipate similar volume and shape retention in upcoming in vivo rat studies. Discussion/Significance of Impact: Incorporating DCM improves in vitro form retention in bioengineered ear cartilage without impeding cell viability, suggesting DCM can act as an internal rebar to resist contraction and preserve geometry. By continuing to advance this construct translationally, next in vivo, we aim to enable its eventual use in clinical ear reconstruction.

Objectives/Goals: To address the disparity in type 2 diabetes within the Latino community, we utilized digital story telling narrative-based videos created by and for individuals with lived experiences. Methods/Study Population: We utilized digital story telling narrative-based videos created by and for individuals with lived experiences. The targeted population is the Latino community. Results/Anticipated Results: Our qualitative results were discordant with the quantitative findings. Rather than four distinct patterns based on self-efficacy and HbA1c levels, we found five common themes across the groups: (1) trauma shapes readiness for change; (2) caregiving responsibilities can hinder self-management; (3) persistence is key to long-term T2D control; (4) social support is essential; and (5) self-advocacy and a desire for knowledge drive success. These results highlight the multifaceted challenges Latino/Hispanic individuals face in managing type 2 diabetes, where overlapping social and cultural factors influence both self-efficacy and health outcomes. Discussion/Significance of Impact: Interventions for Latino/Hispanic communities should be well informed and family/community-centered, and that will strengthen the persistence, support network, and patient empowerment to translate confidence into sustained glycemic control.

Objectives/Goals: Linking GAINS evaluates a 14-week community-based program combining genomic testing, ancestry education, and culturally tailored nutrition to improve cardiometabolic health, literacy, and behavior among African American adults in Baton Rouge, Louisiana. Methods/Study Population: Sixty African American adults in Baton Rouge will be randomized into genetically blinded (GB) or unblinded (GU) groups. Both complete baseline surveys, biometric assessments, and a 14-week health behavior program with screenings, nutrition education, cooking classes, and exercise. The GU group receives Color Genomics testing and counseling at baseline; GB receives counseling post-intervention. Follow-up measures assess literacy, diet, and cardiometabolic outcomes. Results/Anticipated Results: CHATGPT SAID: We anticipate improvements in genomic literacy, nutrition knowledge, and willingness to undergo genetic testing, particularly among the unblinded group. Participants are expected to adopt healthier eating and physical activity behaviors, leading to modest reductions in BMI and blood pressure and increased trust in genomics and preventive health research. Discussion/Significance of Impact: Linking GAINS shows how combining genomic testing with culturally tailored nutrition education can improve literacy and lifestyle behaviors, offering a scalable model for advancing equitable precision health in African American communities.

Objectives/Goals: We aim to determine whether inadequate sphingosine-1-phosphate (S1P) production in hyperglycemia is involved in deranged inflammatory response and host defense during methicillin-resistant Staphylococcus aureus (MRSA) skin infection. Methods/Study Population: Using a Phenome-Wide Association Study (PheWAS), we analyzed a disease-agnostic cohort of ~40,000 individuals to identify disease associations with SNPs in genes involved in the S1P pathway. RNA-seq data enabled comparison of key S1P-related genes in diabetic foot ulcer and non-ulcerated diabetic foot skin. For animal data, we infected euglycemic (EG) and hyperglycemic (HG) mice with MRSA and monitored infection resolution over 9 days. Biopsies were taken on days 1, 3, and 9, and S1P levels, lesion size, bacterial load, and cell death were assessed. To evaluate whether S1P can enhance clearance of dead cells/bacteria and promote healing, we treated mice with FTY720, an S1P agonist FDA-approved for multiple sclerosis Results/Anticipated Results: PheWAS analysis suggests that SGPL1 SNP rs111439207 has protective effects against MRSA infection (OR = 1.71, P = 0.0004) and type 1 diabetes (OR = 1.325,P = 0.003). The RNA-seq dataset revealed increased SGPL1 expression (an enzyme responsible for S1P degradation) in the skin of diabetic patients with foot ulcers. In animals, we show that infected HG mice develop larger lesions, higher bacterial loads, and poor abscess formation. We found a correlation between dead cell buildup and a lower ratio of ingested dead cells in the skin by macrophages of HG mice compared to EG mice. Topical S1P treatment decreased lesion size and bacterial load in HG mice. Discussion/Significance of Impact: Our data show increased expression of SGPL1 in diabetic patients. S1P deficiency during MRSA skin infection leads to tissue damage, uncontrolled bacterial growth, and poor resolution of the infection in mice. Topical treatment with S1P agonist is a promising therapeutic approach to enhance patient healing.