Artificial intelligence (AI) has emerged as one of the most powerful forces shaping clinical and translational sciences. There are real opportunities as well as significant challenges and potential threats, which become very real upon implementation. At Translational Science 2026 in Milwaukee, Wisconsin, the Regulatory Sciences and Clinical Research Professional Taskforce Special Interest Groups (SIGs) jointly discussed opportunities, challenges, and threats associated with AI in clinical and translational sciences through the lived experiences of the SIGs’ members. Common uses, concerns, questions, and gaps were surfaced in the discussions and are presented in this manuscript, along with possible paths forward.