Video games are prevalent and provide interactive reading platforms for learning. Despite its popularity and pedagogical potential, little is known about how players from different language backgrounds read text in video games. This exploratory study recruited 104 participants, including 56 first-language (L1) English speakers and 48 second-language (L2) English learners, who played the tutorial chapter of a commercial video game while their eye movements were recorded. The results showed that, at the subtitle level, L2 players had higher fixation counts and longer saccades than L1 players. At the word level, the word frequency effect was stronger among L2 players than L1 players in both early- and late-stage linguistic processing, as indicated by longer fixations on lower-frequency words. These eye-movement patterns suggest that L2 players adopted a more in-depth and effortful scanning strategy than L1 players when sampling textual information in gameplay. A similar lexical processing pattern was also found in an exploratory analysis of L2 players with different levels of L2 proficiency, further indicating the importance of vocabulary size for efficient lexical processing. The findings provide empirical insights into reading theories and practices related to digital reading.