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15 - Measurements, Estimation, and Control

Published online by Cambridge University Press:  05 April 2013

Henry C. Lim and
Hwa Sung Shin
Henry C. Lim
Affiliation:
University of California, Irvine
Hwa Sung Shin
Affiliation:
Inha University, Seoul
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Summary

To optimize bioreactor operations and to operate them effectively, it is essential to be able to monitor and control the bioreactor operating conditions. It is most desirable to have reliable in situ, on-line sensors and measurement devices that can supply measurements of various variables and parameters without a significant time delay so that the information provided by these devices can be used to implement optimization strategies to improve the productivity and efficiency of processes. These devices can be broadly classified as in situ, on-line, and off-line.

This on-line data acquisition would allow on-line optimization and control of bioreactors. For the purposes of process optimization and control, it is desirable to have reliable in situ sensors with a short response time, and because they come into direct contact with the culture medium, they must withstand the sterilization process. When it is not possible to sterilize the sensors, on-line measurement via sampling of the culture medium or culture gas stream can be utilized. Thus, shorter sampling lines that can maintain sterility are required for the liquid and gas sample lines.

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Chapter
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Fed-Batch Cultures
Principles and Applications of Semi-Batch Bioreactors
 , pp. 407 - 436
Publisher: Cambridge University Press
Print publication year: 2013

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