We use all 27 years of airborne laser altimetry from the University of Alaska Fairbanks to quantify changes in surface elevation across 205 large glaciers that together represent 56% of the glacier area in Alaska and adjacent Canada, including 71% of the ice cover in Alaska’s National Parks. We reprocessed lidar point clouds into a homogenized publicly available 2.5 m digital elevation model (DEM) archive, implemented a new double-differencing approach to incorporate partially overlapping surveys and derived elevation change in 10 m elevation bins to assess glacier-wide mass balance for all surveyed epochs on each glacier. The resulting mean balance rate for all measured glaciers shows statistically significant acceleration of mass loss, ranging from
$-0.83 \pm{0.13} \,\, \text{m}\,\, \text{w.e.}\ \text{a}^{-1}$ before 2008 to
$-1.16 \pm{0.08} \,\, \text{m}\,\, \text{w.e.}\ \text{a}^{-1}$ thereafter. Extrapolating measured thinning rates from the latter period (2008–22) to unsurveyed glaciers suggests that Alaskan National Parks are losing 33.74
$\text{Gt a}^{-1}$ of ice, approximately one third of all estimated losses in the region. We use case studies of several prominent glaciers to show how this sustained acceleration of mass loss, which is consistent with recent global assessments, is having direct impacts on visitors, land managers, ecosystems and geohazards in the National Parks.