Wild bees in agricultural landscapes face multiple stressors, including habitat loss and pesticide exposure. As demand for sustainably produced agricultural products increases, it is essential to understand how sustainable practices influence wild bee communities. Bee responses to such practices were assessed across 26 vineyards in Canada over 2 years. Wild bees were sampled alongside vegetation and floral resources to evaluate the effects of cover cropping, mowing frequency, alternate row management, organic farming, and certified sustainable management on bee abundance, diversity, and community composition. Vegetation height between vine rows was the strongest positive predictor of both bee abundance and diversity, while vegetation cover and floral abundance also had positive associations. A higher proportion of flowers from cover crop species was negatively associated with bee abundance, specifically for pan-trap collected bees. Alternate row and organic management had limited effects on bee abundance and diversity, while certified sustainable management showed a slight negative impact on bee diversity, as well as reduced floral richness and vegetation height. Community composition analyses revealed that both the bee communities and flower communities differed between vineyard management types, though the use of cover cropping and alternate row management did not differ between organic, certified sustainable, and conventional sites. These findings suggest that reduced mowing frequency may be an effective strategy for supporting wild bees, while other sustainable practices, such as cover cropping, organic farming, and certified sustainable management, may require enhanced research approaches to detect effects or further refinements to improve their conservation outcomes.