A meta-analysis was conducted to evaluate the effects of whey protein supplementation on growth performance, nutrient digestibility, blood parameters and carcase quality in broiler chickens. The dataset was compiled from studies published between 1964 and 2025, following the PICO framework, which included the following components: population = broiler chickens; intervention = various whey products (types of whey, inclusion methods and different inclusion levels); comparison = control versus treatment; and outcome = growth performance, nutrient digestibility, carcase traits, humoral immunity, antioxidant properties, intestinal morphology and microbiota composition. Overall, the inclusion of whey protein in broiler diets significantly improved average daily gain, feed intake, breast percentage and villus height (g’ > 0.500; P < 0.001). In conclusion, this meta-analysis highlights the strong potential of whey protein as an alternative protein source in poultry nutrition.

Winter barley is mainly grown in Europe. Before new varieties are recommended for cultivation, they undergo evaluation in breeding and variety trials. Based on the results of these trials, the stability and adaptability of promising new lines or varieties are assessed. In the present study, based on results from post-registration trials, we compared varieties grown in the 2020/21, 2021/22 and 2022/23 seasons. We fitted two Bayesian mixed models and assessed the stability of the varieties using the posterior estimates of variance components from the preferred model. We also used Bayesian probabilistic methods to recommend the best varieties. Using the probabilistic methods, we identified the varieties that were the most stable and had the highest yield in the barley post-registration trials. The varieties Melia, Mirabelle and Zenek were shown to be the three most stable and highest yielding. Furthermore, these three varieties had the highest joint probability of superior performance and stability. This study demonstrates that probabilistic methods within a Bayesian framework are a powerful tool for recommending the best winter barley varieties. The R-codes for both models are provided in a Supplement.

Production efficiency of pasture-based livestock production systems is primarily driven by the level of pasture utilisation, and, as such, regular monitoring of herbage mass (HM) provides essential information to assist on-farm decision making. Unfortunately, this practice is seldom carried out on commercial farms, likely due to the time commitment required across the entire grass-growing season. Recent studies have shown, however, that even moderately inaccurate HM data can improve the system-side profitability compared to enterprises with no data, warranting further investigations into the trade-off between the accuracy and cost associated with HM measurements. Using a weekly multi-paddock dataset from the North Wyke Farm Platform research site in Devon, UK, this study evaluated the technical validity and labour-saving potential of a simplified ‘pasture walk’ protocol for rising plate meters, under which only data along the diagonal transect – rather than the industry-standard W-shaped pathways – of the paddock are collected. Across 234 temporal-paddock combinations, the mean absolute difference in HM estimates between diagonal and W-transects was 106 kg DM/ha, a scale far too small to alter sward or animal management. The presented statistical analysis, together with a supplementary spatial simulation experiment, supported the generality of the findings across the full grass-growing season. With a 51.2% reduction in labour time (1.2 min/ha rather than 2.5 min/ha) across paddocks of various sizes and shapes, the proposed method is likely to facilitate uptake of evidence-based grazing management amongst farmers who currently do not quantify HM at all.

The study analysed corn harvested areas from 1960 to 2030, using data from the USDA open-access official dataset and 1000 autoregressive integrated moving average models. The global corn harvested area experienced a steady growth rate of 1.0 % in the 1960s, followed by a slight setback in the 1980s and a recovery in the 1990s. The early 2000s saw significant expansion, while the 2010s saw a slightly slower pace of 1.4 %. From 2020 to 2024, the global growth rate declined to 0.0 %, suggesting a potential shift towards a more stable or slower growth phase. The most likely scenario for the global corn area is an optimistic outlook with a 37 % likelihood of steady growth. China’s most likely scenario is a gradual expansion with a rate of 2.0 %. The United States’ most likely scenario suggests a neutral trend. Brazil has remarkable growth trajectories in its areas, reflecting land availability. Ukraine’s area by 2030 is projected to experience varying outcomes due to uncertainties in the agricultural landscape.

The objective of this study was to assess the fermentation profile, chemical composition, aerobic stability and taxonomic diversity of corn grain silages rehydrated with water or cactus pear. Two rehydration methods were tested: corn grain silage rehydrated with water (CW) and corn grain silage rehydrated with cactus pear (CCP), each subjected to four opening times (30, 60, 90 and 120 days). The experiment employed a 2 × 4 factorial completely randomized design (two rehydration methods and four opening times) with four repetitions, units 32 experimental units. pH values were higher in water-rehydrated corn grain silage compared to cactus pear-rehydrated silage at 60 (average of 4.78 and 4.33) and 90 days (average of 4.33 and 3.83). For NH3-N, CW surpassed CCP at 30 days (average of 0.73% and 0.63%) and 60 days (average of 1.09% and 0.74%), respectively. Regarding rehydration, CCP had a higher dry matter (DM) content at 30 and 60 days, while CW showed the highest DM content at 90 and 120 days. Initially, the microbiota of CW and CCP treatments differed, primarily in the abundance of the Weissella genus, more abundant in CCP. However, from 30 to 120 days, microbiotas in all treatments became taxonomically similar, with no significant differences. Both silage experienced an increase in bacteria of the Lactobacillus genus. The use of cactus pear for rehydration in ensiling rehydrated corn grain is viable, showing superior results for fermentation profile and aerobic stability compared to water rehydration. It is recommended to open the silo after 60 days of fermentation.

Elephant grass [Cenchrus purpureus (Schum.) Morrone] is a tropical forage grass widely used in ruminant feeding in tropical and subtropical areas, mainly in cut-and-carry systems due to is high potential for herbage accumulation. However, most common genotypes (tall genotypes) generally show an early stem elongation, which contributes to a rapid decline in the forage nutritive value. Dwarf elephant grass types usually present less stem elongation and may be considered an option for the production of high nutritive value forage in cut-and-carry systems. The aims of this study were to evaluate and explain variations in herbage characteristics and kinetics of in vitro gas production of irrigated elephant grass genotypes (two tall-types – Elephant B and IRI-381 – and two dwarf-types genotypes – Mott and Taiwan A-146 2.37) under two harvest frequencies (60 and 90 days) in the dry and rainy seasons of two years. Herbage characteristics varied among genotypes, harvest frequency and seasons. Dwarf genotypes showed better in vitro fermentation parameters with the advancement of maturity compared to the tall size genotypes. Harvesting tall genotypes at a higher frequency (60-day) contributed to improving the fermentation, while dwarf elephant grass maintained greater fermentation when less frequent harvests were adopted (90-day).

Chickpea (Cicer arietinum L.) is a vital legume crop with significant global importance, yet its productivity is highly sensitive to environmental variability. This study employed advanced statistical modelling to identify key environmental drivers of chickpea yield and water-use efficiency (WUE). Field trial data from 29 experiments across 10 Australian locations were analysed, focusing on 19 climatic variables across four growth stages: sowing to flowering, flowering to podding, podding to maturity and the critical period around flowering. Using correlation analysis and Exclusive LASSO regression, the study quantified relationships between environmental factors, growth stages and chickpea performance metrics. Key findings identified soil evaporation and soil moisture supply-demand ratio during the sowing-to-flowering stage, along with frost during the critical period, as significant determinants of yield. Frost negatively impacted WUE across multiple growth stages, while mean photothermal quotient during early growth positively influenced transpiration-based WUE. Predictive models developed using daily climate data demonstrated strong performance (R2 > 0.68–0.72) for yield and WUE predictions. The study provides actionable insights for optimising chickpea production under varying environmental conditions, offering practical tools for farmers and agronomists to enhance crop management strategies, supporting sustainable and profitable chickpea farming in Australia and beyond.

Studies on tillering dynamics are essential to understand the aspects underlying the persistence and adaptation pathways of grass communities, especially in more complex multispecific pastures. This study aimed to assess the tillering dynamics and population stability of Andropogon gayanus cv. Planaltina (PG), Megathyrsus maximus cv. Massai (MG) and Urochloa brizantha cv. BRS Piatã (PP) grown as monocultures and as a mixture. The treatments corresponded to three grasses described above. Sixteen 180 m2 plots were randomly assigned and managed intermittently under manual harvest at pre- and post-harvest heights of 35 and 17.5 cm, respectively, for two years. During autumn/winter/early spring, when resource availability is limited by abiotic factors, pasture population stability was ensured by the conservation strategy of all species, mainly through the high tiller survival rate (85.5 ± 0.32). In late spring and summer, the capture strategy was prioritized for all species, with stability ensured through high tiller appearance rates (30.3 ± 0.80 and 40.4 ± 1.47, respectively), which compensated for the high tiller death rate (28.7 ± 1.10) and resulted in greater species turnover during these both seasons. The association among PG, MG and PP in a mixture allows species to coexist with relatively stable populations and tillering dynamics, similar to their monocultures. These findings represent a step forward in our understanding of mixed swards stability and indicate that such associations could be viable alternatives to palisadegrass monocultures in tropical regions. Further research should test this mixed sward under grazing, during medium to long-term.

Soil health is essential for a resilient ecosystem. The European Union proposed a Soil Monitoring Law for a legal framework of soil health. This study proposes a way to assess the mineral soil health. A database of mineral soils containing <20% organic matter and consisting of 10 soil classes and 22 soil types was used. There were four altitudinal groups (HM [high mountains], LM [low mountains and high hills], LH [low hills], PL [low plains]), covering the vegetation/climate floors, two land uses (forestland and grassland combined and cropland) and three soil textures (coarse [CO], loamy [LO] and clayey [CL]). Both soil organic carbon (SOC)/clay ratios and observed per mean SOC (O/M SOC) ratios were calculated for 19 regions. For SOC/clay, the 1/13, 1/10 and 1/8 thresholds were used, whereas O/M SOC categories were grouped as ‘low’, ‘intermediate’, ‘high’ and ‘very high’ health. SOC/clay and O/M SOC ratios combined were used to characterize soil health. SOC sequestration depends on many factors that are specific for each pedo-climatic region and texture and so is the soil characterization as healthy or not healthy. The recommended simultaneous application of these two indicators revealed specific SOC content values as reference levels for good soil health, which decrease from the wetter climates towards the drier ones. SOC content considerably differed among pedo-climatic regions, and soil health should be compared within the same regions that have specific SOC sequestration conditions. Correlations between support points SOC values and the aridity index allow separation between ‘healthy’ and ‘non-healthy’ soil conditions for any climate, vegetation floor and land use.

This study evaluated the effects of combining Bacillus species with hydrolysed and inactive Torula yeast on rumen fermentation of tropical forages in vitro. Exp. 1 assessed different combinations of direct-fed microbials (DFM) and hydrolysed/inactive yeast on the ruminal fermentation of Urochloa brizantha hay in a randomised block design (3×3 factorial scheme). Treatments included: 1) Negative control, no DFM inclusion (NC); 2) B. subtilis and B. licheniformis (SL); 3) B. subtilis, B. licheniformis, B. amyloliquefaciens and B. coagulans (SLAC); 4) Hydrolysed Torula yeast (HY); 5) dry inactive Torula yeast (IY); 6) B. subtilis, B. licheniformis and dry inactive Torula yeast (SL+IY); 7) B. subtilis, B. licheniformis and hydrolysed Torula yeast (SL+HY); 8) B. subtilis, B. licheniformis, B. amyloliquefaciens, B. coagulans and dry inactive Torula yeast (SLAC+IY) and 9) B. subtilis, B. licheniformis, B. amyloliquefaciens, B. coagulans and hydrolysed Torula yeast (SLAC+HY). Bacillus and yeast interaction affected the total short-chain fatty acids (SCFA) and SCFA profile. In treatments without Bacillus, IY resulted in higher isovalerate compared to treatments without yeast (NC). The no yeast treatment also had lower isovalerate concentration than other treatments (HY, IY). Exp. 2 and Exp. 3 evaluated NC, SL, SLAC, SL+HY and SLAC+IY plus SLAC+HY in low- and medium-quality forage. For low-quality forage, SLAC+IY + SLAC+HY had the highest NH3-N concentration, while SL+HY yielded the highest total SCFA. In medium-quality forage, SL+HY showed the highest total SCFA. The combination of Bacillus species and HY may enhance total SCFA and NH3-N in the rumen, improving nutrient utilisation in grazing beef cattle.

Appropriate soil water and nitrogen (N) management strategies are critical for achieving sustainable agricultural development in drylands. Straw mulching has been used to improve crop yield and water use efficiency (WUE), but N management strategies may need to be adjusted from conventional practice. The current study investigated the interactive effects of N application rate (conventional and high N rate), N application frequencies (single, and split N in 2 – 3 applications) and seasonal conditions on wheat population density dynamics, yield, harvest index (HI), grain protein content, water- and N-use efficiency, and residual soil N under straw mulching on the Loess Plateau of China. Nitrogen rate had no effect on yield, HI, WUE and grain protein content, but high N rate resulted in lower grain weight and nitrogen partial factor productivity (PFPN), and higher soil N residue. Splitting N applications significantly improved grain yield (7%), HI (9%), grain protein content (5%), PFPN and N harvest index, along with a reduction in soil N residue, compared to single application. However, there was no difference in above traits between split-N in 2 and 3 applications. Conventional N rate (vs. high N rate) and split N application (vs. single application) both alleviated the negative correlation between grain yield and grain protein content, and split N application increased grain N removal per unit yield compared to single N application. It is concluded that conventional N rate combined with split application in two doses, is suitable for straw mulching in drylands of the Loess Plateau, China.

Canola (Brassica napus L.) is an important oilseed crop with notable economic and nutritional value; however, its productivity in newly reclaimed soils is hindered by nutrient deficiencies and complex soil conditions. This study examined the combined effects of magnesium (Mg) fertilisation and zinc (Zn) foliar applications on the growth, yield and oil quality of canola in newly reclaimed soils in Egypt during the 2021/2022 and 2022/2023 growing seasons. A split-plot experimental design was utilised, featuring Mg rates (12, 24, 48 and 72 kg/ha) as main plots and Zn foliar applications (0, 3, 5 and 6 g/L) as sub-plots. The results indicated significant improvements in plant growth characteristics, yield components and oil parameters with elevated levels of Mg and Zn. The treatment combination of 72 kg Mg/ha and 6 g/L Zn consistently improved plant height, number of primary branches, siliques per plant and 1000-seed weight in both seasons. Seed yield increased by 71%, achieving 3.50 t/ha, while oil yield exhibited a 134.9% rise, reaching 1,377.43 kg/ha. Principal component analysis revealed that the increased application of Mg and Zn significantly enhanced agronomic performance, with the first two principal components accounting for 90.7% of the total variance. Correlation and path analyses revealed intricate interactions among traits, with the number of siliques per plant and oil content demonstrating the most significant direct effects on seed and oil yields. This study highlights the significance of balanced nutrient management for optimising canola productivity.

To manage anaerobic digestion residues (digestate) sustainably, it is important to determine their agricultural properties. In the present study, the effects of two digestate fractions (solid and liquid) on processing tomato yield parameters, quality traits, health-related compounds and some fruit physiological disorders were evaluated. The solid and liquid digestate fractions were compared with chemically fertilized and unfertilized control to evaluate the potential of the digestate as a fertilizer. A 2-year experiment was conducted in a randomized complete block design, with three replications and using two tomato varieties: cv. ‘Arte F1’ and cv. ‘Zeplin F1’. The results indicated that (1) compared with chemical fertilizer, the solid digestate produced equal or even better results in terms of fruit size, yield parameters (solid digestate treatment increased the total fruit weight per plant by an average of 30.7, 8.2 and 22.4% in 2019 and 25.3, 14.2 and 17.9% in 2022 compared with control, chemical fertilizer and liquid treatments, respectively) and percentage of fruit affected by sunscald and blossom-end rot in both years; (2) use of liquid digestate led to similar or significantly higher fruit size, yield parameters and percentage of fruit affected by sunscald and blossom-end rot than control in both years and (3) use of both solid and liquid digestate fractions significantly maintained or improved fruit quality in terms of colour traits, pericarp thickness, dry matter content, total soluble solid content, titratable acidity, pH, vitamin C and antioxidant activity. However, the effects of solid and liquid digestate fractions varied with year and variety.

Cephalaria joppensis Rchb. (CJ) has recently been domesticated as a new forage crop in Israel. Feeding trials with dairy cows and sheep have shown that CJ silage can replace wheat silage for these animals. The current work tested the effect of row spacing on the growth, yield, chemical composition and digestibility of CJ for forage. Two field trials were performed at a research farm in Acre to test three row spacings: (T1) 16 cm between rows (narrow row spacing), (T2) pairs of adjacent (16 cm apart) rows with 32 cm spacing between pairs (alternated row spacing) and (T3) 32 cm between rows (wide row spacing). T1 row spacing had the advantage of a better area coverage rate, which may be beneficial at the beginning of growth, reflected by the rapid establishment of the plants in the field, as well as in the competition with weeds. The growth rate was more significant in T2 and T3 row spacings. In the first year, a higher yield was obtained for T2 and T3 row spacings; in the second year, no differences in yield were found among the row spacing treatments. Row spacing did not affect the plant’s digestibility or chemical nutrition for ruminants. There was no uniformity in plant quality among the cultivars between the two years. Thus, we should define the phenological stages of Cephalaria joppensis and their relationship to nutritional quality at different sites having different climate conditions, in both small and semi-commercial plots.

The facilities where the livestock carry their production cycle must have as their main characteristic the control of the influence of variation of environmental factors on the animals, which can be controlled through the use of different ventilation systems. In this way, the objective of this research was to evaluate the influence of different conditioning systems in the swine farrowing house. Three treatments were tested in a farrowing house located in Parana, Brazil: refrigerated ventilation (T1), forced ventilation (T2) and natural ventilation (T3), on 12 females with 11 piglets each. The climatic parameters evaluated were temperature, velocity and relative humidity of the air. For the females were analysed the parameters: surface temperature (laser thermometer and thermal image), average weight and feed conversion at the end of lactation; and for the piglets: surface temperature (thermal image), average weight and weekly feed conversion. The data were interpreted statistically by analysis of variance and Tukey’s test with 5% probability of error, using the program SAS (2008). The use of the refrigerated ventilation system decreased the temperature of the air and the superficial temperature of the females, without affecting the comfort of the piglets. There was no difference in the productive indexes of the animals, thus other aspects of the production chain could be explored to reflect a higher financial gain.

The presence of excessive arsenic (As) in paddy fields poses a significant risk to human health due to its accumulation in rice grains. However, the current level of As in Sri Lankan paddy fields remains unclear. Therefore, this research aims to assess the distribution of exchangeable As concentration and investigate the effects of agro-climatic zones (ACZs), soil orders and water sources on exchangeable As concentration in Sri Lankan paddy fields. For this purpose, 7,154 soil samples were collected from paddy fields using a stratified random sampling method representing six ACZs, six soil orders and three water sources. Arsenic extraction was made using 0.01 M CaCl2 followed by the detection with inductive coupled plasma mass spectrophotometry. The concentration of exchangeable As ranged from 0.01 to 392.9 µg/kg with an average of 24.6 µg/kg. Samples from the Low-country Wet zone exhibited higher exchangeable As levels compared to those from the Low country Dry zone (P < 0.05). Among soil orders, Histosols, Inceptisols and Ultisols showed higher exchangeable As concentrations than Alfisols and Vertisols (P < 0.05). Rainfed paddy fields had higher exchangeable As compared to the fields with access to supplementary irrigation. Additionally, exchangeable As concentration was inversely correlated with soil pH and paddy grain yield (P < 0.05). The observed variations in soil-As concentration across ACZs, soil orders and water sources highlight the need for climate, soil order and water source-specific strategies to mitigate further accumulation of As in paddy fields.

Extreme weather events caused by climate change, such as drought and heavy rainfall, will further increase in Central Europe in the near future. Resilient crop production requires in-depth knowledge of soil moisture (SM), its spatial and temporal variability and the dynamics of agriculturally used land. In the current study, different SM estimation methods, including measurement and simulation-based methods, were evaluated over a 17-ha experimental arable crop field with respect to their abilities to capture the spatial and temporal SM dynamics of within-field areas and their related uncertainty and spatial representativeness. The high-spatial resolution in-situ topsoil moisture measurements (50 m grid) were compared with the estimated SM from satellite-based remote sensing (S1ASCAT) and the simulated SM from three different crop water balance models (Agricultural Risk Information System [ARIS], AquaCrop and DSSAT). The evaluation revealed that the spatial variability in the experimental field obtained from the reference could not be captured by the alternative methods investigated because of the limitations of the grid size-related soil map information. Nevertheless, the analysis revealed a very good temporal correlation of SM dynamics with the field area average across all approaches, with AquaCrop and ARIS at a soil depth of 0–10 cm and S1ASCAT soil–water index 05 achieving a R2 and a Kling–Gupta efficiency >0.80. These results indicate the added value of complementary methods for estimating SM to reduce spatial and temporal uncertainties in the estimated topsoil water content.